Mineralization of 2,4,6-trinitrophenol (picric acid): Characterization and phylogenetic identification of microbial strains
Mineralization of 2,4,6-trinitrophenol (picric acid): Characterization and phylogenetic identification of microbial strains
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DOI：
10.1007/bf01570041
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发表时间：
1996-05-01
期刊：
影响因子：
--
通讯作者：
Pace, NR
中科院分区：
文献类型：
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作者：
Rajan, J;Valli, K;Pace, NR
分离到4株以苦味酸为唯一碳源和能源的细菌，C-14-UL-苦味酸的矿化表明，高达65%的放射性以（CO_2）-C-14形式释放，HPLC和UV/维斯光谱分析表明，这些细菌完全降解苦味酸。HPLC和LC/MS分析表明，在苦味酸降解过程中瞬时形成2，4-二硝基苯酚。苦味酸的降解伴随着每摩尔苦味酸释放3摩尔亚硝酸盐的化学计量释放。基于小亚基（16 S）rRNA基因序列，将四种苦味酸降解剂鉴定为简单诺卡氏菌（ATCC 6946）的近亲。
Four bacterial strains that use picric acid as their sole carbon and energy source were isolated, Mineralization of C-14-UL-picric acid showed that up to 65% of the radioactivity was released as (CO2)-C-14, HPLC and UV/Vis spectral analyses indicated complete degradation of picric acid by these organisms. HPLC and LC/MS analyses showed transient formation of 2,4-dinitrophenol during picric acid degradation. Degradation of picric acid was concomitant with stoichiometric release of three moles of nitrite per mole of picric acid, The four picric acid degraders were identified as close relatives of Nocardioides simplex (ATCC 6946) based on their small subunit (16S) rRNA gene sequences.