DIVISION INHIBITION GENE DICF OF ESCHERICHIA-COLI REVEALS A WIDESPREAD GROUP OF PROPHAGE SEQUENCES IN BACTERIAL GENOMES
DIVISION INHIBITION GENE DICF OF ESCHERICHIA-COLI REVEALS A WIDESPREAD GROUP OF PROPHAGE SEQUENCES IN BACTERIAL GENOMES
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DOI：
10.1128/jb.176.4.1150-1156.1994
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发表时间：
1994-02-01
影响因子：
3.2
通讯作者：
BOUCHE, JP
中科院分区：
文献类型：
--
作者：
FAUBLADIER, M;BOUCHE, JP
通过Southern印迹杂交分析各种真细菌的基因组，以检测与编码DicF RNA（大肠杆菌K-12中的细胞分裂基因ftsZ的反义抑制剂）的缺陷型类芽孢杆菌原噬菌体Kim的片段相关的序列。在所发现的同源序列中，有一个来自E. coli B中与Rac原噬菌体相似的一个片段和福氏志贺菌中的两个片段进行了克隆和测序。在每个序列中都发现了类似于转录终止子的dicF样元件，但与dicF不同，这些元件对E. coli K-12。像dicF一样，这些序列的侧翼是二级结构，形成了RNase III识别的潜在位点。编码序列位于上游的dicF样功能在E。coli B与噬菌体h的sieB基因相关，而S.弗氏元件与卫星噬菌体P4的免疫区相似。在杂交条件下，只有很强的序列同源性检测E。coli B和S.福氏杆菌，包括一些革兰氏阳性细菌在内的多种微生物的基因组，与dicF探针杂交。我们的研究结果表明，dicF和它的侧翼区域是一个广泛的家庭的不同来源的原噬菌体样元件的标记。
The genomes of various eubacteria were analyzed by Southern blot hybridization to detect sequences related to the segment of the defective lambdoid prophage Kim which encodes DicF RNA, an antisense inhibitor of cell division gene ftsZ in Escherichia coli K-12. Among the homologous sequences found, one fragment from E. coli B, similar to a piece of Rac prophage, and two fragments from Shigella flexneri were cloned and sequenced. dicF-like elements similar to transcriptional terminators were found in each sequence, but unlike dicF these had no effect on division in E. coli K-12. Like dicF, these sequences are flanked by secondary structures which form potential sites for RNase III recognition. Coding sequences located upstream from the dicF-like feature in E. coli B are related to gene sieB of bacteriophage h, while sequences downstream of the S. flexneri elements are similar to the immunity region of satellite bacteriophage P4. Under hybridization conditions in which only strong sequence homologies were detected in E. coli B and S. flexneri, the genomes of a large variety of microorganisms, including some gram-positive bacteria, hybridized to the dicF probe. Our results suggest that dicF and its flanking regions are markers of a widespread family of prophage-like elements of different origins.