A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis
A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis
复制标题
集成多室聚合酶链式反应和多通道分离的遗传分析微流控装置
DOI：
10.1016/j.aca.2010.06.005
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发表时间：
2010-07-26
影响因子：
6.2
通讯作者：
Qin, Jianhua
中科院分区：
文献类型：
--
作者：
Pan, Xiaoyan;Jiang, Lei;Qin, Jianhua
这项工作描述了一种集成多室聚合酶链式反应 (PCR) 和多通道分离的微流体装置，用于并行遗传分析。该微装置由三个功能单元组成：温度控制、多重PCR（四室PCR）和多通道分离（四个分离通道，每个通道连接到一个PCR室）。采用铂（Pt）/钛（Ti）微加热器确保温度场均匀，并采用Pt芯片传感器进行温度监测。通过将PCR室与分离通道直接连接，简化了芯片PCR和芯片分离之间的接口。芯片PCR后，PCR产物被引入平行分离通道，通过自动施加电场进行后续分离/检测。该微器件成功应用于乙型肝炎病毒（HBV）和结核分枝杆菌（MTB）等病原体的检测以及人类白细胞抗原（HLA）-B27的基因分型，证明了集成微器件用于并行遗传分析的可行性。 (C) 2010 Elsevier B.V. 保留所有权利。
This work describes a microfluidic device integrated with multichamber polymerase chain reaction (PCR) and multichannel separation for parallel genetic analysis. The microdevice consists of three functional units: temperature control, multiple PCR (four chambers PCR), and multiple channel separation (four separation channels, each channel connected to a PCR chamber). Platinum (Pt)/titanium (Ti) microheater was used to ensure homogeneous temperature field, and Pt-chip sensor was used for temperature monitoring. The interface between chip-PCR and chip separation was simplified by connecting the PCR chamber with separation channel directly. After chip-PCR, PCR products were introduced into parallel separation channels for subsequent separation/detection by applying an electric field automatically. This microdevice was successfully applied for detection of pathogens including hepatitis B virus (HBV) and Mycobacterium tuberculosis (MTB), and genotyping of human leucocyte antigen (HLA)-B27 as well, demonstrating the feasibility of the integrated microdevice for parallel genetic analysis. (C) 2010 Elsevier B.V. All rights reserved.