A radioimmunoassay for 18-hydroxycortisol in plasma and urine.
A radioimmunoassay for 18-hydroxycortisol in plasma and urine.
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血浆和尿液中 18-羟基皮质醇的放射免疫测定。
DOI：
10.1093/clinchem/31.6.849
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发表时间：
1985
影响因子：
9.3
通讯作者：
P. Budd
中科院分区：
文献类型：
--
作者：
J. Corrie;C. Edwards;P. Budd
18-羟基皮质醇排泄增加已被提议作为鉴别诊断原发性醛固酮增多症的特异性生化标志物。我们描述了一种直接RIA的发展与125 I标记，允许测量类固醇在小于或等于0.5微升的尿液或小于或等于25微升的血浆。对于对照受试者，尿和血浆中18-羟基皮质醇的平均浓度分别为310（SD 178）nmol/24 h（n = 32）和2.27（SD 0.80）nmol/L（n = 37）; Conn腺瘤或糖皮质激素可治疗的醛固酮增多症患者的尿数值范围为1084至6534 nmol/L。24 h，血浆浓度范围为6.49 ~ 31.20 nmol/L。特发性肾小球增生患者的尿液和血浆值分别为353 - 734 nmol/24 h和0.26 - 6.60 nmol/L。尿中18-羟基皮质醇浓度明显区分特发性增生患者与其他形式的原发性醛固酮增多症，但需要进一步的工作，以评估血浆中测定的诊断准确性。
Increased excretion of 18-hydroxycortisol has been proposed as a specific biochemical marker for differential diagnosis of primary aldosteronism. We describe the development of a direct RIA with an 125I label that permits measurement of the steroid in less than or equal to 0.5 microL of urine or less than or equal to 25 microL of plasma. For control subjects, the mean concentrations of 18-hydroxycortisol in urine and plasma were 310 (SD 178) nmol/24 h (n = 32) and 2.27 (SD 0.80) nmol/L (n = 37), respectively; patients with Conn's adenoma or glucocorticoid-remediable aldosteronism had values for urine in the range 1084 to 6534 nmol/24 h and concentrations in plasma ranging from 6.49 to 31.20 nmol/L. Patients with idiopathic zona glomerulosa hyperplasia had values for urine and plasma ranging from 353 to 734 nmol/24 h and from 0.26 to 6.60 nmol/L, respectively. Concentrations of 18-hydroxycortisol in urine clearly discriminate patients with idiopathic hyperplasia from those with other forms of primary aldosteronism, but further work is required to assess the diagnostic accuracy of determinations in plasma.
18-氧化皮质醇，一种天然存在的盐皮质激素激动剂。
影响因子：
4.8
作者：
Ulick,S;Land,M;Chu,MD
通讯作者：
Chu,MD