FINE-STRUCTURE OF THE CHITIN PROTEIN SYSTEM IN THE CRAB CUTICLE
FINE-STRUCTURE OF THE CHITIN PROTEIN SYSTEM IN THE CRAB CUTICLE
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DOI：
10.1016/0040-8166(84)90020-x
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发表时间：
1984-01-01
期刊：
影响因子：
2.6
通讯作者：
GIRAUDGUILLE, MM
中科院分区：
文献类型：
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作者：
GIRAUDGUILLE, MM
通过透射电子显微镜观察，岸蟹角质层 (C. maenas L.) 有机基质的精细结构揭示了几丁质-蛋白质复合物的 3 种不同组织水平。最高级别对应于 Bouligand (1972) 描述的扭曲胶合板组织。水平微纤维平行于角质层平面，逐渐从一个水平旋转到另一水平。当从斜切面观察时，该结构在所有几丁质蛋白质层中呈现出一系列叠加的嵌套弧，在光学显微镜或电子显微镜的最低放大倍数下可见。在电子显微镜的最高放大倍数和最佳分辨率下，当超薄切片恰好横向于微纤维时，可以观察到由对电子透明的棒组成的恒定图案，这些棒嵌入不电子透明的基质中。在横截面中，这些杆通常形成或多或少的六边形阵列。微纤维是一根棒，相邻的不透明材料是另一根。对微纤维分子结构的通常解释受到质疑。在这两个组织层次之间，有一个中间层次，对应于微原纤维的分组。微原纤维形成致密结构，膜层（角质层的最深层且非钙化层）中几乎没有自由空间。在角质层的其他部分，微原纤维聚集成各种直径的原纤维或形成网状结构，有机基质的自由空间被矿物质占据。
The fine structure of the organic matrix of the shore crab cuticle (C. maenas L.), observed in transmission electron microscopy, reveals 3 different levels of organization of the chitin-protein complex. The highest level corresponds to the twisted plywood organization described by Bouligand (1972). Horizontal microfibrils, parallel to the cuticle plane, rotate progressively from one level to another. When viewed in oblique section this structure gives superimposed series of nested arcs, visible in light microscopy or at the lowest magnifications of the EM, in all the chitin-protein layers. At the highest magnifications of the EM and with the best resolution, when the ultrathin sections are exactly transverse to the microfibril, a constant pattern can be observed which consists of rods transparent to electrons, which are embedded in an electron-opaque matrix. In cross-section, these rods often form more or less hexagonal arrays. A microfibril is one rod and the adjacent opaque material is another. The usual interpretation of the microfibril molecular structure is questioned. Between these 2 levels of organization, there is an intermediate level, which corresponds to the grouping of microfibrils. Microfibrils form a dense structure, with few free spaces in the membranous layer, the deepest and non-calcified layer of the cuticle. In other parts of the cuticle, microfibrils are grouped into fibrils of various diameters or form a reticulate structure, the free spaces of the organic matrix are occupied by the mineral.