Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay
Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay
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DOI：
10.1515/cclm.2004.257
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发表时间：
2004-01-01
影响因子：
6.8
通讯作者：
Böger, RH
中科院分区：
文献类型：
--
作者：
Schulze, F;Wesemann, R;Böger, RH
不对称二甲基精氨酸（ADMA）是一种内源性竞争性一氧化氮合酶（NOS）抑制剂。已报道ADMA血浆水平升高与内皮L-精氨酸-NO途径受损和内皮功能障碍相关的疾病有关，如动脉粥样硬化、高胆固醇血症、慢性心力衰竭、糖尿病和高血压。由于ADMA水平升高，NOS产生的NO减少。事实上，越来越多的人对测定不同来源样品中的ADMA水平感兴趣。本工作的目的是开发一种精确和简单的免疫测定方法，与现有的方法相比，如HPLC，液相色谱-质谱（LC-MS）和气相色谱（GC）-MS。
Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide synthase (NOS). Elevated ADMA plasma levels have been reported in connection with diseases associated with an impaired endothelial L-arginine-NO pathway and endothelial dysfunction, such as atherosclerosis, hypercholesterolemia, chronic heart failure, diabetes mellitus, and hypertension. NO production by NOS is decreased due to elevated ADMA levels. In fact, there is increasing interest in determination of ADMA levels in samples of various origins. The aim of this work was to develop a precise and easy immunoassay in contrast to the existing methods, such as HPLC, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS. We determined cross-reactivity in our immunoassay of 1.2% for symmetric dimethylarginine and