Urethan (ethyl carbamate) alone is carcinogenic for mouse skin.
Urethan (ethyl carbamate) alone is carcinogenic for mouse skin.
复制标题
单独的尿烷（氨基甲酸乙酯）对小鼠皮肤具有致癌作用。
作者：
O. Iversen
将9组32只无毛小鼠（每组16只雄性和16只雌性）的背部皮肤涂上约200微升的聚氨酯溶液，每周两次，持续58周。给予以下处理：单独的丙酮、单独的丙二醇、丙酮中10%的氨基甲酸乙酯、丙二醇中15%的氨基甲酸乙酯、丙酮中20%的氨基甲酸乙酯、丙二醇中30%的氨基甲酸乙酯、丙酮中40%的氨基甲酸乙酯、丙二醇中75%的氨基甲酸乙酯和大约100%的熔融氨基甲酸乙酯。组织学检查未发现一般表皮增生或炎症反应。皮肤、肝脏和淋巴结中出现肿瘤，一只小鼠的肾脏和肺部也出现白血病浸润。记录皮肤肿瘤的发生率和产量并进行统计学分析。还记录了发生的其他肿瘤的数量。结果证实，氨基甲酸乙酯是肺和其他几个器官的肿瘤原，它也是无毛小鼠皮肤的完全致癌物质，即使在丙酮中的剂量低至10%的氨基甲酸乙酯。还存在显著的剂量-反应关系。
Nine groups of 32 hairless mice (16 males and 16 females in each) were painted on the back skin with approximately 200 microliters solution of urethan twice a week for up to 58 weeks. The following treatments were given: acetone alone, propylene glycol alone, 10% urethan in acetone, 15% urethan in propylene glycol, 20% urethan in acetone, 30% urethan in propylene glycol, 40% urethan in acetone, 75% urethan in propylene glycol, and approximately 100% melted urethan. Histological examinations revealed no development of general epidermal hyperplasia or inflammatory reactions. Tumors developed in skin, liver and lymph nodes, and one mouse also developed leukemic infiltrations in kidneys and lungs. The rate and yield of skin tumors were registered and statistically analyzed. The number of other tumors occurring was also recorded. The results confirm that urethan is a tumorigen for the lung and several other organs, and that it is also a complete carcinogen for hairless mouse skin, even in a dose as low as 10% urethan in acetone. There is also a significant dose-response relationship.