INAPPARENT VIRUS-INFECTIONS AND THEIR INTERACTIONS IN PUPAE OF THE HONEY BEE (APIS-MELLIFERA LINNAEUS) IN AUSTRALIA
INAPPARENT VIRUS-INFECTIONS AND THEIR INTERACTIONS IN PUPAE OF THE HONEY BEE (APIS-MELLIFERA LINNAEUS) IN AUSTRALIA
复制标题
DOI：
10.1099/0022-1317-69-7-1617
复制
发表时间：
1988-07-01
影响因子：
3.8
通讯作者：
GIBBS, AJ
中科院分区：
文献类型：
--
作者：
ANDERSON, DL;GIBBS, AJ
当来自看似健康的澳大利亚蜂群的蜜蜂蛹被注射各种盐溶液时，黑蜂王细胞病毒（BQCV）、克什米尔蜜蜂病毒（KBV）、囊胞病毒（SBV）以及偶尔的蟋蟀麻痹病毒的隐性感染被激活。蛹在35℃下孵育后，激活的病毒复制到可检测的浓度。 C 3天。仅通过在 35 摄氏度下孵化蛹也可以激活 SBV 的隐性感染，但其他病毒则不然。 C. 通过向蛹注射针对每种病毒颗粒的兔抗血清，可以特异性抑制激活的 BQCV、KBV 或 SBV 的复制。然而，激活的 SBV 感染发生在激活的 KBV 复制受到抑制的蛹组中，比未抑制 KBV 的蛹组中发生的比例更高。此外，与未注射KBV和SBV抗血清的蛹组相比，在KBV和SBV复制均受到抑制的蛹中发生激活的BQCV感染的比例更高。以这种方式处理的一组蛹的结果表明，约 40% 感染 KBV，约 43% 感染 SBV，约 15% 感染 BQCV。没有证据表明病毒之间在建立蛹的隐性感染过程中存在干扰，但有明确的证据表明在激活期间或之后存在干扰。这些结果对于利用蛹进行病毒传播具有重要意义。
When honey bee pupae from seemingly healthy Australian colonies were injected with various salt solutions, inapparent infections of black queen-cell virus (BQCV), Kashmir bee virus (KBV), sacbrood virus (SBV) and, occasionally, cricket paralysis virus were activated. The activated viruses replicated to detectable concentrations after pupae were incubated at 35.degree. C for 3 days. Inapparent infections of SBV, but not the other viruses, were also activated merely by incubating pupae at 35.degree. C. The replication of activated BQCV, KBV or SBV was specifically suppressed by injecting pupae with rabbit antisera against the particles of each virus. However, a greater proportion of activated SBV infections occurred in groups of pupae in which the replication of activated KBV was suppressed than in groups of pupae in which KBV was not suppressed. In addition, a greater proportion of activated BQCV infections occurred in pupae in which the replication of both KBV and SBV was suppressed than in groups of pupae that were not injected with KBV and SBV antisera. Results from one group of pupae treated in this way indicated that about 40% were infected with KBV, about 43% with SBV and about 15% with BQCV. There was no evidence of interference between the viruses in the establishment of inapparent infections of pupae, but clear evidence of interference during or after activation. These results have implications for the use of pupae for virus propagation.