Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients
Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients
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DOI：
10.1099/vir.0.80882-0
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发表时间：
2005-06-01
影响因子：
3.8
通讯作者：
Ares, BR
中科院分区：
文献类型：
--
作者：
Baré, P;Massud, I;Ares, BR
为了研究丙型肝炎病毒（HCV）在血友病患者外周血单个核细胞（PBMC）中的持续存在和复制，研究了HCV单感染和HIV/HCV共感染患者PBMC培养上清液（SN）中HCV的产生和释放。HCV RNA（+）SN在免疫重建较差的HIV/HCV合并感染者中的检出率（89.5%）高于单纯HCV感染者（57%）或对高效抗逆转录病毒治疗有良好反应的HIV/HCV合并感染者（50%）。培养SN中HCV基因组的存在与较低的CD 4（+）T细胞计数和更严重的HIV感染临床表现相关。尽管长期阴性的HCV病毒血症，从HIV/HCV共感染患者的PBMC释放HCV基因组培养后。HCV容许PBMC允许产生具有连续HCV复制的HCV生产性B细胞系。这些发现进一步加重了PBMC参与HCV感染的持续性，并强调了B淋巴细胞作为HCV储存库的作用。
In order to investigate hepatitis C virus (HCV) persistence and replication in peripheral blood mononuclear cells (PBMC) from a group of haemophilic individuals, HCV production and release to PBMC culture supernatants (SNs) from HCV singly infected patients and HIV/HCV co-infected patients was studied. HCV RNA(+) SNs were found more frequently from HIV/HCV co-infected individuals (89.5%) with poor reconstitution of their immune status than from singly HCV-infected patients (57%) or from HIV/HCV co-infected individuals with a good response to highly active anti-retroviral therapy (50%). The presence of the HCV genome in culture SNs was associated with lower CD4(+) T-cell counts and with a more severe clinical picture of HIV infection. In spite of prolonged negative HCV viraemia, PBMC from HIV/HCV co-infected patients released the HCV genome after culture. HCV permissive PBMC allowed generation of HCV productive B cell lines with continuous HCV replication. These findings add further weight to the involvement of PBMCs in persistence of HCV infection and emphasize the role of B lymphocytes as HCV reservoirs.