Leonurine improves ischemia-induced myocardial injury through antioxidative activity
Leonurine improves ischemia-induced myocardial injury through antioxidative activity
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DOI：
10.1016/j.phymed.2010.01.018
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发表时间：
2010-08-01
期刊：
影响因子：
7.9
通讯作者：
Zhu, Y. Z.
中科院分区：
文献类型：
--
作者：
Liu, X. H.;Pan, L. L.;Zhu, Y. Z.
本研究旨在观察益母草中的化合物4-胍基-正丁基戊酸酯（leonurine）对大鼠缺血心脏的保护作用，以探讨其对大鼠缺血心脏的保护作用机制。结扎大鼠左冠状动脉造成心肌缺血。测定血浆肌酸激酶（CK）、乳酸脱氢酶（LDH）及心脏匀浆超氧化物歧化酶（SOD）活性。益母草碱能显著降低血浆LDH和CK活性。这一观察结果与结扎左冠状动脉引起的缺血大鼠心脏梗死面积减少相一致。逆转录聚合酶链反应（RT-PCR）检测到益母草碱处理组促凋亡基因Bax的mRNA表达下调0.68倍（p <0.05），抗凋亡基因Bcl-2的mRNA表达上调1.41倍（p < 0.05）。Western blotting检测Bcl-2和Bax蛋白表达水平与基因表达水平相一致。此外，抗氧化酶Mn-SOD的mRNA表达水平显著增加了1.23倍（p < 0.05），这一发现与所观察到的SOD活性增加以及脂质过氧化作用的降低相对应。综上所述，我们的研究结果表明，益母草碱减轻心肌损伤在MI通过抗氧化和抗凋亡作用和益母草碱可能成为一个有用的辅助心脏保护剂。(C)2010年Elsevier GmbH。All rights reserved.
The present study was designed to investigate the protective effects of 4-guanidino-n-butyl syringate (leonurine), a compound in Herba Leonuri (HL) on ischemic rat heart to determine the protective mechanisms associated with ischemic rat hearts. Rat heart ischemia was induced by ligation of the left coronary artery. Creatine kinase (CK) and lactate dehydrogenase (LDH) in plasma and superoxide dismutase (SOD) activity in heart homogenates were measured. We found leonurine significantly decreased levels of LDH and CK activities in plasma. This observation corresponded with decreased infarct size of ischemic rat heart induced by ligation of the left coronary artery. Moreover, the mRNA expression of the pro-apoptotic gene Bax was significantly down-regulated by 0.68-fold (p < 0.05) and the anti-apoptotic gene Bcl-2 was up-regulated by 1.41-fold (p < 0.05) in the leonurine treated groups as compared with acute myocardium ischemia (MI) controls measured by RT-PCR. Correspondingly, Bcl-2 and Bax protein levels detected by Western blotting coincided with gene expression levels. In addition, the mRNA expression level of the antioxidant enzyme Mn-SOD was significantly increased 1.23-fold (p < 0.05) and this finding corresponded with an observed increase in SOD activity and also with a committed decrease in lipid peroxidation. Taken together, our results demonstrated that leonurine attenuated myocardium injury during MI via antioxidative and anti-apoptotic effects and leonurine might become a useful adjuvant cardioprotective agent. (C) 2010 Elsevier GmbH. All rights reserved.