Altered expression of BDNF and its high-affinity receptor TrkB in response to complex motor learning and moderate exercise
Altered expression of BDNF and its high-affinity receptor TrkB in response to complex motor learning and moderate exercise
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DOI：
10.1016/j.brainres.2004.09.003
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发表时间：
2004-11-26
期刊：
影响因子：
2.9
通讯作者：
Greenough, WT
中科院分区：
文献类型：
--
作者：
Klintsova, AY;Dickson, E;Greenough, WT
我们报告说，配合复杂的运动技能或适度运动的大鼠，小脑和运动皮质中脑源性神经营养因子(BDNF)及其受体TrkB蛋白的表达发生了变化。现已知道，体力活动会增加某些神经营养因子的表达。我们在1、3、5、7或14天后检测了以下三种情况之一的BDNF和TrkB表达的时间进程：(1)杂技运动技能组合条件(AC)，(2)运动活动条件(在平坦的跑道上中等速度跑；MC)和(3)作为基线组的不活跃的社交控制(SC)组。通过测定免疫细胞化学反应产物的相对光密度来评价BDNF和TrkB的表达水平。在小脑分子层，BDNF的表达与训练前7d在AC和MC中的停留时间显著相关，在AC训练14d后持续升高，而在MC训练中则无明显变化。小脑分子层TrkB蛋白表达的变化与BDNF在训练前7天的变化相一致，但随后其表达降至对照水平。在运动皮质，AC后14d，BDNF和TrkB蛋白在上层的表达显著增加。14天后，BDNF在上运动皮质层的表达增加，而TrkB的表达未见增加。这些数据表明，复杂的运动捆绑和适度的体力活动与较少的捆绑会对BDNF及其受体的表达模式产生不同的影响，并可能对此类经历产生的神经可塑性产生影响。(C)2004爱思唯尔B.V.保留所有权利。
We report that rats teaming complex motor skills or exercising moderately show changes in expression of brain-derived neurotrophic factor (BDNF) and its receptor, TrkB protein, in cerebellum and motor cortex. It is now known that physical activity increases expression of some neurotrophins. We examined the time course of BDNF and TrkB expression after 1, 3, 5, 7 or 14 days in one of three conditions: (1) an "acrobatic" motor skill teaming condition (AC), (2) a motor activity condition (moderately paced running on a flat track; MC) and (3) an inactive social-only control (SC) that served as a baseline group. Expression levels of BDNF and TrkB were evaluated by measuring relative optical density of the immunocytochemical reaction product. In cerebellar molecular layer, expression of BDNF correlated significantly with time spent in AC and MC over the first 7 days of training and remained elevated after 14 days of AC but not of MC. Changes in TrkB protein expression in cerebellar molecular layer mirrored those for BDNF during the first 7 days of training, but subsequently its expression subsided to the control level. In motor cortex, a significant increase in BDNF and TrkB protein expression was detected in the upper layers after 14 days in AC. Increased expression of BDNF, but not of TrkB, was observed in upper motor cortical layers after 14 days of MC. These data indicate that complex motor teaming and moderate physical activity with little teaming produce different effects on the expression pattern of BDNF and its receptor and may have implications for neural plasticity arising from such experiences. (C) 2004 Elsevier B.V. All rights reserved.