Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation
Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation
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DOI：
10.1016/j.febslet.2012.03.008
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发表时间：
2012-04-24
期刊：
影响因子：
3.5
通讯作者：
Hong, Jeong-Ho
中科院分区：
文献类型：
--
作者：
Byun, Mi Ran;Kim, A. Rum;Hong, Jeong-Ho
骨质疏松症源于成骨细胞骨形成和破骨细胞骨吸收之间的失衡。在这项研究中，我们从海洋天然产物中筛选能刺激成骨细胞分化的分子。我们发现佛波醇酮A显著刺激间充质细胞中的成骨细胞分化。TAZ和Runx2相互作用增强刺激了佛波醇酮A诱导的成骨细胞标记基因的表达。ERK的激活对于分化的刺激很重要，因为ERK抑制剂阻断了佛波醇酮A诱导的成骨分化。综上所述，结果表明佛波醇酮A通过激活ERK刺激TAZ介导的成骨细胞分化。
蛋白质相互作用的结构化总结：TAZ通过下拉实验与RUNX2发生物理相互作用（查看相互作用） (C) 2012欧洲生物化学学会联合会。由爱思唯尔B.V.出版。保留所有权利。
Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK.Structured summary of protein interactions:TAZ physically interacts with RUNX2 by pull down (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.