Recognition imaging with a DNA aptamer
Recognition imaging with a DNA aptamer
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DOI：
10.1529/biophysj.105.079111
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发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Lindsay, Stuart
中科院分区：
文献类型：
--
作者：
Lin, Liyun;Wang, Hongda;Lindsay, Stuart
我们已经使用了DNA适配体拴在原子力显微镜探针进行识别成像的IgE分子附着在云母基板。识别是有效的（类似于90%）和特异性的，通过注射溶液中的IgE分子来阻断，并且不受高浓度的第二种蛋白质的干扰。识别信号的信噪比比用抗体获得的识别信号的信噪比好，尽管断裂适体-蛋白质键所需的平均力略小。
We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (similar to 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.