Stimulation of ornithine decarboxylase activity in chick fibroblasts by non‐suppressible insulin‐like activity (NSILA), insulin and serum
Stimulation of ornithine decarboxylase activity in chick fibroblasts by non‐suppressible insulin‐like activity (NSILA), insulin and serum
复制标题
通过不可抑制的胰岛素样活性 (NSILA)、胰岛素和血清刺激鸡成纤维细胞中的鸟氨酸脱羧酶活性
DOI：
10.1002/jcp.1040880213
复制
发表时间：
1976
影响因子：
5.6
通讯作者：
R. Humbel
中科院分区：
文献类型：
--
作者：
G. Haselbacher;R. Humbel
一种从人血清中分离的因子（非抑制性胰岛素样活性，NSILA）刺激血清饥饿鸡胚成纤维细胞的增殖，并刺激鸟氨酸脱羧酶（ODC）的活性。生理剂量的NSILA（200 IU/ml）和药理剂量的胰岛素（200 IU/ml）刺激ODC 4-5倍，10%胎牛血清刺激约18倍。NSILA和胰岛素的组合添加不会导致更高的活性，表明共同的作用机制。用NSILA、胰岛素或血清获得的细胞数量的增加与ODC刺激的程度平行。用链霉蛋白酶处理细胞也刺激ODC活性。
A factor isolated from human serum (nonsuppressible insulin‐like activity, NSILA) stimulates multiplication of serum‐starved chick embryo fibroblasts and stimulates activity of ornithine decarboxylase (ODC). Physiological doses of NSILA (200 m̈ U/ml) and pharmacological doses of insulin (200 m U/ml) stimulate ODC 4–5‐fold, 10% fetal calf serum about 18‐fold. Combined addition of NSILA and insulin does not result in higher activities, suggesting a common mechanism of action. The increase in cell number obtained with NSILA, insulin or serum parallels the degree of ODC stimulation. Treatment of cells with pronase also stimulates ODC activity.