Heterogeneity and dynamics of lateral line afferent innervation during development in zebrafish (Danio rerio)
Heterogeneity and dynamics of lateral line afferent innervation during development in zebrafish (Danio rerio)
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DOI：
10.1002/cne.22798
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发表时间：
2012-05-01
影响因子：
2.5
通讯作者：
Liao, James C.
中科院分区：
文献类型：
--
作者：
Haehnel, Melanie;Taguchi, Masashige;Liao, James C.
斑马鱼幼鱼的侧线系统正成为研究神经生物学中从毛细胞再生到感觉加工等一系列课题的模型。然而，尽管许多研究详细说明了侧线神经瘤的模式和发育，但对它们与传入神经元和后脑靶点的连接组织知之甚少。我们发现，随着鱼的生长和神经瘤在体表的增殖，传入神经元的数量呈线性增加。支配某些神经瘤的传入神经的数量随着时间的推移而增加，而其他神经瘤的传入神经的数量则减少。传入神经元的比例，神经丘之间的前，后侧线系统不同，这表明在敏感性阈值或空间分辨率的潜在差异。一个单一的传入神经元经常接触一组神经肥大，这表明不同的传入神经元可以沿着沿着身体传递感受野的信息。当传入投射被追踪到后脑，在那里一个独特的somatotopy先前已被描述，我们发现，这种一般的组织是不存在的毛特纳细胞。我们推测，从侧线方向输入是不太重要的，在早期的年龄，而速度的逃避反应是至关重要的，方向性的反应出现在以后的发展。通过量化侧线系统中的形态连接，本研究提供了一个详细的基础，以了解如何处理流体动力学信息，并最终转化为适当的运动行为。神经学比较杂志520：13761386，2012. (c)2011 Wiley Periodicals，Inc.
The lateral line system of larval zebrafish is emerging as a model to study a range of topics in neurobiology, from hair cell regeneration to sensory processing. However, despite numerous studies detailing the patterning and development of lateral line neuromasts, little is known about the organization of their connections to afferent neurons and targets in the hindbrain. We found that as fish grow and neuromasts proliferate over the body surface, the number of afferent neurons increases linearly. The number of afferents innervating certain neuromasts increases over time, while it decreases for other neuromasts. The ratio of afferent neurons to neuromasts differs between the anterior and posterior lateral line system, suggesting potential differences in sensitivity threshold or spatial resolution. A single afferent neuron routinely contacts a group of neuromasts, suggesting that different afferent neurons can convey information about receptive fields along the body. When afferent projections are traced into the hindbrain, where a distinct somatotopy has been previously described, we find that this general organization is absent at the Mauthner cell. We speculate that directional input from the lateral line is less important at an early age, whereas the speed of the escape response is paramount, and that directional responses arise later in development. By quantifying morphological connections in the lateral line system, this study provides a detailed foundation to understand how hydrodynamic information is processed and ultimately translated into appropriate motor behaviors. J. Comp. Neurol. 520:13761386, 2012. (c) 2011 Wiley Periodicals, Inc.