RAS2 of Saccharomyces cerevisiae is required for gluconeogenic growth and proper response to nutrient limitation.
RAS2 of Saccharomyces cerevisiae is required for gluconeogenic growth and proper response to nutrient limitation.
复制标题
酿酒酵母的 RAS2 是糖异生生长和对营养限制的适当反应所必需的。
DOI：
10.1073/pnas.82.11.3785
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发表时间：
1985
影响因子：
11.1
通讯作者：
Breitenbach,M
中科院分区：
文献类型：
--
作者：
Tatchell,K;Robinson,LC;Breitenbach,M
酿酒酵母含有两个与ras癌基因家族成员具有显著同源性的基因。这两个基因，RAS1和RAS2，构成了一个必需的基因家族，因为这两个基因的破坏孢子不能生长。我们在这里报告，含有RAS2中断的菌株有三种不同的表型。首先，它们不能在不可发酵的碳源上有效生长。第二，它们过度积累储存碳水化合物糖原和海藻糖。第三，对于RAS2破坏纯合的二倍体细胞在丰富培养基上形成孢子。已经分离出了抑制基因缺陷的基因外抑制因子。这些抑制因子至少分为三个互补组，其中两个突变绕过RAS对细胞活力的正常要求，允许不含RAS基因的细胞生长。RAS 2突变体和基因外抑制因子的表型表明RAS在营养限制的正常反应中具有某些功能。
Saccharomyces cerevisiae contains two genes with remarkable homology to members of the ras oncogene family. These two genes, RAS1 and RAS2, constitute an essential gene family since spores with disruptions of both genes fail to grow. We report here that strains containing RAS2 disruptions have three distinct phenotypes. First, they fail to grow efficiently on nonfermentable carbon sources. Second, they hyperaccumulate the storage carbohydrates glycogen and trehalose. Third, diploid cells homozygous for the RAS2 disruptions sporulate on rich media. Extragenic suppressors have been isolated that suppress the gluconeogenic defect. These suppressors fall into at least three complementation groups, mutations in two of which bypass the normal requirement of RAS for cell viability, allowing cells containing neither RAS gene to grow. The phenotype of the RAS2 mutant and extragenic suppressors implicate RAS with some function in the normal response to nutrient limitation.