INTERLEUKIN-6 IS AN AUTOCRINE GROWTH-FACTOR FOR MESANGIAL CELLS
INTERLEUKIN-6 IS AN AUTOCRINE GROWTH-FACTOR FOR MESANGIAL CELLS
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DOI：
10.1038/ki.1990.193
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发表时间：
1990-08-01
影响因子：
19.6
通讯作者：
COLEMAN, DL
中科院分区：
文献类型：
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作者：
RUEF, C;BUDDE, K;COLEMAN, DL
白细胞介素6（interleukin 6，IL-6）可诱导急性期反应、B细胞分化、T细胞、胸腺细胞、造血祖细胞、杂交瘤细胞和浆细胞瘤细胞增殖。单核细胞、T细胞、成纤维细胞、上皮细胞和内皮细胞分泌IL-6。由于IL-6反应细胞类型可能参与肾小球炎症的发病机制，我们研究了IL-6的分泌大鼠MC，使用IL-6依赖性杂交瘤细胞系B9。我们的研究结果表明，MCs分泌IL-6，分子量为17 - 42 kDa，等电点为4.0至5.3。MC-IL-6活性可被抗IL-6多克隆抗体阻断。北方印迹法检测MC表达IL-6 mRNA。此外，我们的数据表明，IL-6作为MC的自分泌生长因子。用重组IL-6孵育亚融合MC导致3H-胸苷掺入和MC数量的剂量依赖性增加。此外，含有IL-6活性的MC-CM的反相HPLC级分增加MC的3H-胸苷掺入。MC-IL-6除了可能在介导肾小球免疫应答中发挥旁分泌作用外，还可能是导致体内系膜细胞增殖的自分泌信号之一。
Interleukin 6 (IL-6) induces the acute phase response, differentiation of B cells, proliferation of T cells, thymocytes, hematopoietic progenitors, hybridoma and plasmacytoma cells. Monocytes, T cells, fibroblasts, epithelial and endothelial cells secrete IL-6. Since IL-6 responsive cell-types may participate in the pathogenesis of glomerular inflammation, we studied the secretion of IL-6 by rat MCs, using the IL-6 dependent hybridoma cell line B9. The results of our studies indicate that MCs secrete IL-6 with a molecular weight of 17-42 kDa and isoelectric point of 4.0 to 5.3. MC-IL-6 activity could be blocked by a polyclonal antimurine-IL-6 antibody. MC express IL-6 mRNA as determined by Northern blot. Furthermore, our data demonstrate that IL-6 acts as an autocrine growth factor for MC. Incubation of subconfluent MC with recombinant IL-6 results in a dose-dependent increase of 3H-thymidine incorporation and number of MCs. Moreover, reverse phase HPLC fractions of MC-CM containing IL-6 activity increase 3H-thymidine incorporation by MC. In addition to its possible paracrine role in mediating the immune response in the glomerulus, MC-IL-6 may also be one of the autocrine signals leading to mesangial cell proliferation in vivo.