Yarrowia lipolytica Pex20p, Saccharomyces cerevisiae Pex18p/Pex21p and mammalian Pex5pL fulfil a common function in the early steps of the peroxisomal PTS2 import pathway
Yarrowia lipolytica Pex20p, Saccharomyces cerevisiae Pex18p/Pex21p and mammalian Pex5pL fulfil a common function in the early steps of the peroxisomal PTS2 import pathway
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DOI：
10.1093/embo-reports/kve228
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发表时间：
2001-11-01
期刊：
影响因子：
7.7
通讯作者：
Schliebs, W
中科院分区：
文献类型：
--
作者：
Einwächter, H;Sowinski, S;Schliebs, W
过氧化物酶体基质蛋白的输入对过氧化物酶体的生物发生至关重要。利用各种不同模型系统的遗传和生化研究已经发现了这一过程所需的23个PEX基因。尽管人们普遍认为，与线粒体和叶绿体不同，蛋白质转运到过氧化物酶体中涉及一个受体循环，但据报道，在不同生物体中，这个循环的进化保护在成分或步骤方面存在差异。我们在这里表明，蛋白质进入过氧化物酶体的早期步骤表现出比以前认为的更大的相似性。脂解耶氏菌的Pex20p、酿酒酵母的Pex18p和Pex21p以及哺乳动物的Pex5pL在各自生物的PTS2通路中发挥着共同的功能。这些非同源蛋白具有一个保守的序列区域，很可能代表一个常见的pts2受体结合位点和可能参与对接蛋白结合的二芳香五肽基序。我们认为，在过氧化物酶体蛋白质输入的早期阶段，不一定是相同的蛋白质，但它们的功能模块是保守的。
Import of peroxisomal matrix proteins is essential for peroxisome biogenesis. Genetic and biochemical studies using a variety of different model systems have led to the discovery of 23 PEX genes required for this process. Although it is generally believed that, in contrast to mitochondria and chloroplasts, translocation of proteins into peroxisomes involves a receptor cycle, there are reported differences of an evolutionary conservation of this cycle either with respect to the components or the steps involved in different organisms. We show here that the early steps of protein import into peroxisomes exhibit a greater similarity than was thought previously to be the case. Pex20p of Yarrowia lipolytica, Pex18p and Pex21p of Saccharomyces cerevisiae and mammalian Pex5pL fulfil a common function in the PTS2 pathway of their respective organisms. These non-orthologous proteins possess a conserved sequence region that most likely represents a common PTS2-receptor binding site and di-aromatic pentapeptide motifs that could be involved in binding of the putative docking proteins. We propose that not necessarily the same proteins but functional modules of them are conserved in the early steps of peroxisomal protein import.