Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.
Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.
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使用荧光交联剂稳定肌球蛋白亚片段 1 中的初级环。
DOI：
10.1073/pnas.82.6.1658
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发表时间：
1985
影响因子：
11.1
通讯作者：
Morales,MF
中科院分区：
文献类型：
--
作者：
Mornet,D;Ue,K;Morales,MF
双功能荧光烷基化剂二溴摩烷已被用于稳定肌球蛋白亚片段1 （S-1）中预先存在的初级环。所获得的交联将20-kDa结构域（以前称为“20K”结构域）的Cys-707（称为巯基“SH1”）与50-kDa结构域的硫醇连接起来，似乎将二溴莫烷置于atp可摄动色氨酸附近。
A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain and seems to place the dibromobimane near the ATP-perturbable tryptophan.