Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing.
Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing.
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DOI：
10.1016/j.bios.2014.03.067
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发表时间：
2014-10
影响因子：
12.6
通讯作者：
Zhao Zhang;Liqiang Luo;Guifang Chen;Yaping Ding;D. Deng;C. Fan
中科院分区：
文献类型：
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作者：
Zhao Zhang;Liqiang Luo;Guifang Chen;Yaping Ding;D. Deng;C. Fan
提出了一种合成色胺官能化还原氧化石墨烯(TRA-rGO)的新方法，用于制备无标记电化学阻抗DNA生物传感器。TrA通过π堆积作用与其吲哚环固定在RGO上。用原子力显微镜、高分辨电子显微镜、循环伏安法、电化学阻抗谱(EIS)、拉曼光谱和荧光光谱对TRA-rGO的形貌、导电性和相互作用进行了表征。将氨基取代的寡核苷酸探针通过交联剂戊二醛偶联到TRA上，制备了电化学生物传感平台。EIS检测寡核苷酸探针与靶DNA的杂交反应。在最佳条件下，该传感器对互补寡核苷酸的检测具有较高的灵敏度和较低的检测下限。目标寡核苷酸的定量范围为1 0×10−12~1 0×10−7M，检出限为5 2×10−13M(S/N=3)。
A novel simple protocol of synthesizing tryptamine-functionalized reduced graphene oxide (TRA–rGO) was proposed to fabricate label-free electrochemical impedance DNA biosensor. TRA was anchored to rGO with its indole ring via π-stacking interaction. The morphology, conductivity and interaction of TRA–rGO were characterized by atomic force microscopy, high resolution transmission electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) as well as Raman and fluorescence spectroscopy. The amino-substituted oligonucleotide probe was conjugated to TRA by cross-linker glutaraldehyde for preparing an electrochemical biosensing platform. The DNA hybridization reaction of oligonucleotide probe with target DNA was monitored by EIS. Under optimum conditions, the proposed biosensor exhibited high sensitivity and low detection limit for detecting complementary oligonucleotide. The target oligonucleotide could be quantified in a wide range of 1.0×10−12–1.0×10−7M with low detection limit of 5.2×10−13M (S/N=3).