Bone morphogenetic protein signaling in rotator cuff muscle atrophy and fatty infiltration.
Bone morphogenetic protein signaling in rotator cuff muscle atrophy and fatty infiltration.
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DOI：
10.32098/mltj.02.2015.10
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发表时间：
2019-01
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影响因子：
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通讯作者：
Xuhui Liu;S. Joshi;B. Ravishankar;D. Laron;Hubert T. Kim;B. Feeley
中科院分区：
文献类型：
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作者：
Xuhui Liu;S. Joshi;B. Ravishankar;D. Laron;Hubert T. Kim;B. Feeley
背景：肩袖肌肉的体积减少（萎缩）和脂肪含量增加（脂肪浸润）是大或大量肩袖撕裂的常见并发症，即使在肌腱修复后也被认为是不可逆转的。在临床上，肌肉萎缩和脂肪浸润是导致肌腱修复术后功能不良的重要因素。RC肌萎缩和FI的分子机制尚不明确。在这项研究中，我们利用大鼠模型研究骨形态发生蛋白（BMP）信号在RC肌萎缩和脂肪浸润中的作用。方法采用大鼠单侧大面积RC撕裂法。在损伤后2周和6周收获RC肌肉进行BMP信号分析。在另一个实验中，通过每日腹腔注射给大鼠注射BMP抑制剂（LDN-193189）。在损伤后6周，取实验组和对照组大鼠的RC肌肉进行生化和组织学分析。结果我们发现，经过RCT后，BMP-14和BMP-7在肩袖肌肉中的表达显著增加。抑制BMP信号导致RC撕裂后肌肉萎缩增加，脂肪浸润减少。结论BMP信号抑制RC肌萎缩，但促进脂肪浸润。
BACKGROUND reduced mass (atrophy) and increased fat content (fatty infiltration) of rotator cuff muscles are common complications of large or massive rotator cuff (RC) tears, and are believed to be irreversible even after tendon repairs. Clinically, both muscle atrophy and fatty infiltration are important factors contributing to poor functional outcomes after tendon repairs. The molecular mechanism of RC muscle atrophy and FI remains undefined. In this study, we investigated the role of bone morphogenetic proteins (BMP) signaling in RC muscle atrophy and fatty infiltration using a rat model. METHODS unilateral massive RC tears was induced in adult rats. RC muscles were harvested at 2 and 6 weeks after injury for BMP signaling analysis. In a separate experiment, BMP inhibitor (LDN-193189) was injected to rats through daily intraperitoneal injection. RC muscles from rats in the treated and control groups were harvested at 6 weeks after injury for biochemistry and histology analysis. RESULTS we found significantly increased BMP-14 and BMP-7 expression in rotator cuff muscles after RCT. Inhibiting BMP signaling resulted in increased muscle atrophy and reduced fatty infiltration in rotator cuff muscle after RC tears. CONCLUSION this result suggests that BMP signaling inhibits RC muscle atrophy but promotes fatty infiltration.