3-D magnetic graphene oxide-magnetite poly(vinyl alcohol) nanocomposite substrates for immobilizing enzyme
3-D magnetic graphene oxide-magnetite poly(vinyl alcohol) nanocomposite substrates for immobilizing enzyme
复制标题
DOI：
10.1016/j.polymer.2018.06.046
复制
发表时间：
2018-08-01
期刊：
影响因子：
4.6
通讯作者：
Guo, Zhanhu
中科院分区：
文献类型：
--
作者：
Li, Yanyun;Jing, Tao;Guo, Zhanhu
成功制备了三维磁性氧化石墨烯-磁铁矿聚乙烯醇（3D-GO/PVA/Fe3O4）纳米复合材料。通过扫描电镜（SEM）和透射电镜（TEM）对其形貌进行表征和分析。利用x射线粉末衍射（XPS）、傅里叶变换红外光谱（FT-IR）和x射线衍射光谱（XRD）对其化学结构和晶体结构进行了研究。用振动样品磁强计（VSM）测定了材料的磁性能。比表面积和平均孔径采用brunauer - emmet - teller （BET）法和Barrett-Joyner-Halenda （BJH）法测定。3D-GO/PVA/Fe3O4纳米复合材料的比表面积和平均孔径分别为388.87 m(2)g（-1）和9.6 nm，较高的比表面积表明三维结构避免了氧化石墨烯薄片的聚集。纳米复合材料的饱和磁化强度高达30.5 emu/g，使其易于循环。3D-GO/PVA/Fe3O4纳米复合材料具有较好的猪胰脂肪酶（PPL）固定化性能。固定化后的3D-GO/PVA/Fe3O4纳米复合材料的固定化效率最高达91%，达到其活性的90%。经过10次循环后，固定化酶的活性仍保持在初始活性的70.8%左右。稳定性试验表明，固定化酶在4℃条件下保持56 d的活性可达71.1%。(C) 2018年Elsevier Ltd.出版
Three-dimensional magnetic graphene oxide-magnetite polyvinyl alcohol (3D-GO/PVA/Fe3O4) nano-composites were successfully prepared. The morphology was characterized and analyzed through scanning electron microscope (SEM) and transmission electron microscope (TEM). The chemical structure and the crystal structure were explored by X-ray powder diffraction (XPS), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction spectra (XRD). The magnetic property was obtained by vibrating sample magnetometer (VSM). The specific surface area and the average pore size were determined by Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH). The specific surface and the average pore size of 3D-GO/PVA/Fe3O4 nanocomposites were 388.87 m(2)g(-1) and 9.6 nm, and the higher specific surface indicated that the three-dimensional structure avoided the aggregation for GO sheets. The large saturation magnetization (M-s) of the nanocomposites of 30.5 emu/g enabled the easy cycling of the nanocomposites. The 3D-GO/PVA/Fe3O4 nanocomposites exhibited better performance for porcine pancreatic lipase (PPL) enzyme immobilization. The maximum immobilization efficiency was 91%, and the enzyme immobilized 3D-GO/PVA/Fe3O4 nanocomposites reached up to 90% of their activities. After 10 cycles of reuse, the activity of immobilized enzyme remained about 70.8% of the initial activity. The stability test revealed that the activity of immobilized enzyme remained up to 71.1% at 4 degrees C for 56 days. (C) 2018 Published by Elsevier Ltd.