INCREASED EXPRESSION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR GENE IN MALIGNANT GLIOMAS IS INVARIABLY ASSOCIATED WITH GENE AMPLIFICATION
INCREASED EXPRESSION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR GENE IN MALIGNANT GLIOMAS IS INVARIABLY ASSOCIATED WITH GENE AMPLIFICATION
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DOI：
10.1073/pnas.84.19.6899
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发表时间：
1987-10-01
影响因子：
11.1
通讯作者：
VOGELSTEIN, B
中科院分区：
文献类型：
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作者：
WONG, AJ;BIGNER, SH;VOGELSTEIN, B
对 63 名患者的原发性恶性神经胶质瘤进行了分析，以确定编码表皮生长因子受体 (EGFR) 的基因扩增与相应 mRNA 表达之间的关系。发现 24 个肿瘤扩增了 EGFR 基因，另外 3 个肿瘤中也出现了其他基因的扩增。与合成 RNA 探针的杂交用于原位定量 mRNA 水平。所有 24 个 EGFR 基因扩增的肿瘤都有该基因的高表达水平，而 39 个未扩增的肿瘤中没有一个表达水平升高。这表明，在人类神经胶质瘤中，EGFR 基因表达的大幅增加总是与基因结构的改变相关。
Primary malignant gliomas from 63 patients were analyzed to determine the relationship between amplification of the gene encoding the epidermal growth factor receptor (EGFR) and expression of the corresponding mRNA. Twenty-four tumors were found to have amplified the EGFR gene and amplification of other genes occurred in three additional tumors. Hybridization with synthetic RNA probes was used to quantitate mRNA levels in situ. All 24 tumors with amplification of the EGFR gene had high levels of expression of this gene, while none of the 39 tumors without amplification had increased levels. This shows that, in human gliomas, large increases in the expression of the EGFR gene are invariably associated with alterations in gene structure.