Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices.
Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices.
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DOI：
10.1038/srep16206
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发表时间：
2015-11-10
影响因子：
4.6
通讯作者：
Singamaneni S
中科院分区：
文献类型：
--
作者：
Tadepalli S;Kuang Z;Jiang Q;Liu KK;Fisher MA;Morrissey JJ;Kharasch ED;Slocik JM;Naik RR;Singamaneni S
金属纳米结构的局域表面等离子体共振(LSPR)对吸附的敏感性使其成为一类强大的无标记生物传感器。等离子体纳米结构的光学性质取决于其几何特征和局域介电环境。等离子体纳米换能器表面灵敏度的指数衰减要求在其设计中仔细考虑，特别注意识别层和分析层的大小。在这项研究中，我们证明了短肽作为生物识别元件(BRE)与较大的抗体作为靶标捕获剂相比具有几个优点。使用生物浆纸装置(BPD)，我们展示了心脏生物标记物肌钙蛋白I(CTnI)的选择性和灵敏检测。较小尺寸的多肽使用BPD提供更高的灵敏度和更低的检测下限。此外，基于多肽的LSPR传感器具有优异的保质期和热稳定性，不需要特殊的存储条件，因此非常适合在资源有限的环境中使用。
The sensitivity of localized surface plasmon resonance (LSPR) of metal nanostructures to adsorbates lends itself to a powerful class of label-free biosensors. Optical properties of plasmonic nanostructures are dependent on the geometrical features and the local dielectric environment. The exponential decay of the sensitivity from the surface of the plasmonic nanotransducer calls for the careful consideration in its design with particular attention to the size of the recognition and analyte layers. In this study, we demonstrate that short peptides as biorecognition elements (BRE) compared to larger antibodies as target capture agents offer several advantages. Using a bioplasmonic paper device (BPD), we demonstrate the selective and sensitive detection of the cardiac biomarker troponin I (cTnI). The smaller sized peptide provides higher sensitivity and a lower detection limit using a BPD. Furthermore, the excellent shelf-life and thermal stability of peptide-based LSPR sensors, which precludes the need for special storage conditions, makes it ideal for use in resource-limited settings.