Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes
Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes
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DOI：
10.1016/j.jphotobiol.2006.02.012
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发表时间：
2006-09-01
影响因子：
5.4
通讯作者：
Walterova, Daniela
中科院分区：
文献类型：
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作者：
Psotova, Jitka;Svobodova, Alena;Walterova, Daniela
UVA辐射引起活性氧（ROS）的产生，在暴露的细胞中诱导氧化应激，导致广泛的细胞损伤和细胞凋亡或坏死。保护人体皮肤免受紫外线辐射有害影响的一种方法是使用草药化合物作为光保护剂。本研究评价了夏枯草及其主要酚酸成分迷迭香酸（RA）对uva诱导的人角质形成细胞系（HaCaT）变化的保护作用。暴露于UVA （10-30 J/cm(2)）下的人角质形成细胞，分别用紫荆提取物（1-75 mg/l）或RA提取物（0.9-18 mg/l）处理4小时。紫荆和RA能够减少UVA引起的细胞活力下降，通过中性红保留和LDH释放到培养基中来监测。荆芥提取物和RA显著抑制ltva诱导的ROS生成，表现为细胞内脂质过氧化降低、ATP升高和谷胱甘肽减少。后用凡草提取物和RA处理也显著降低了DNA损伤。此外，uva诱导的caspase-3的激活被寻常紫檀和RA抑制。黄芪提取物和RA具有浓度依赖性的光保护作用（最大剂量分别为25 ~ 50 mg/l和9 mg/l）。这些结果表明，在护肤化妆品中使用的寻常草和RA可能对uva诱导的氧化应激有保护作用，并且可能是光防护皮肤制剂的有益补充。(c) 2006 Elsevier B.V.版权所有
UVA radiation provokes the generation of reactive oxygen species (ROS), which induce oxidative stress in the exposed cells leading to extensive cellular damage and cell death either by apoptosis or necrosis. One approach to protecting human skin against the harmful effects of UV radiation is by using herbal compounds as photoprotectants. This study evaluated the protective effects of Prunella vulgaris L. (Labiatae) and its main phenolic acid component, rosmarinic acid (RA), against UVA-induced changes in a human keratinocyte cell line (HaCaT). Human keratinocytes exposed to UVA (10-30 J/cm(2)) were treated with an extract of P. vulgaris (1-75 mg/l) or RA (0.9-18 mg/l) for 4 h. P. vulgaris and RA exhibited ability to reduce the UVA-caused decrease in a cell viability monitored by neutral red retention and by LDH release into medium. The P. vulgaris extract and RA significantly suppressed LTVA-induced ROS production, which manifests as a decrease in intracellular lipid peroxidation, elevation of ATP and reduced glutathione. Post-treatment with P. vulgaris extract and RA also significantly reduced DNA damage. In addition, UVA-induced activation of caspase-3 was inhibited by treatment with P. vulgaris and RA.The P. vulgaris extract and RA demonstrated a concentration-dependent photoprotection (maximum at 25-50 mg/l and 9 mg/l, respectively). These results suggest that P. vulgaris and RA, used in skin care cosmetics, may offer protection against UVA-induced oxidative stress and may be beneficial as a supplement in photoprotective dermatological preparations. (c) 2006 Elsevier B.V. All rights reserved.