Exolytic products of alginate by the immobilized alginate lyase confer antioxidant and antiapoptotic bioactivities in human umbilical vein endothelial cells
Exolytic products of alginate by the immobilized alginate lyase confer antioxidant and antiapoptotic bioactivities in human umbilical vein endothelial cells
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DOI：
10.1016/j.carbpol.2020.116976
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发表时间：
2021-01-01
影响因子：
11.2
通讯作者：
Zhu, Yanbing
中科院分区：
文献类型：
--
作者：
Jiang, Zedong;Zhang, Xiwen;Zhu, Yanbing
褐藻酸是褐藻中丰富的天然多糖资源，利用褐藻酸裂解酶可将褐藻酸裂解成褐藻酸寡糖。海藻酸裂解酶和具有生物活性的海藻酸寡糖已被应用于药物治疗和营养补充剂等不同领域。固定化酶由于其可重复使用性、稳定性和可调节性，极大地促进了其工业应用。在这项研究中，磁性Fe3O4纳米粒子的合成，并用于包裹的胞外海藻酸裂解酶AlgL17，其特征在于先前。固定化的AlgL17表现出增强的耐热性和pH耐受性，延长的储存稳定性，和适度的可重复使用性。质谱分析表明，固定化AlgL17具有从海藻酸多糖中释放海藻酸寡糖（AOS）的活性。所产生的AOS在H2O2应激的人脐静脉内皮细胞中通过上调活性氧自由基清除活性和减弱半胱天冬酶介导的凋亡途径表现出其抗氧化和抗凋亡活性。
Alginate is a natural polysaccharide resource abundant in brown algae and it can be cleaved into alginate oligosaccharides by alginate lyase. Alginate lyases and the bioactive alginate oligosaccharides have been applied in diverse fields such as pharmaceutical therapy and nutraceutical supplementation. Immobilized enzymes greatly facilitate their industrial application owing to their reusability, stability, and tunability. In this study, magnetic Fe3O4 nanoparticles were synthesized and used to immobilize an exolytic alginate lyase AlgL17 that was characterized previously. The immobilized AlgL17 demonstrated enhanced thermal and pH tolerance, extended storage stability, and moderate reusability. The mass spectrum indicated the specific activity of the immobilized AlgL17 to release alginate oligosaccharides (AOS) from alginate polysaccharide. The produced AOS exhibited their antioxidant and antiapoptotic activities in H2O2-stressed human umbilical vein endothelial cells by upregulation of reactive oxygen species scavenging activities and attenuation of the caspase-mediated apoptosis pathway.