Effect of Panax notoginseng saponins on the pharmacokinetics of aspirin in rats
Effect of Panax notoginseng saponins on the pharmacokinetics of aspirin in rats
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DOI：
10.1016/j.jchromb.2016.12.007
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Zhang, Qiang
中科院分区：
文献类型：
--
作者：
Tian, Zhihao;Pang, Huanhuan;Zhang, Qiang
阿司匹林(ASA)在临床上被广泛用于治疗发热、疼痛、炎症和脑梗塞。三七总皂苷(PNS)是三七(PN)的提取物，是一种广泛用于心血管疾病的中药。三七总皂苷和三七总皂苷是中国教授治疗脑梗塞的常用药物。两药合用在临床上取得了良好的效果。为探讨三七总皂苷对三七总皂苷的体内作用，采用UPLC-MS/MS法测定三七总皂苷口服液及其与三七总皂苷合用后的血药浓度。样品制备采用蛋白质沉淀法，以柴胡皂苷A为内标。采用ACQUITY UPLC(R)BEH C18色谱柱(1.7亩m 2.1×100 mm)，以水(含0.2%甲酸)-乙腈(含0.2%甲酸)为流动相，流速0.2mL/min，实现两组分的分离。药动学参数采用非房室分析方法确定。结果表明，三七总皂甙与阿司匹林存在体内药物相互作用。当两种药物合用时，SA浓度升高。阿司匹林和水杨酸在MDCK-MDR1细胞单层的转运实验证实了这一结论。两药合用时，表观渗透系数(PAPP)值明显增加。提示三七总皂甙可促进ASA和SA在胃肠道的吸收。这些发现为明智地使用两种药物治疗或预防心血管疾病提供了更多的洞察力。(C)2016爱思唯尔B.V.保留所有权利。
Aspirin (ASA) is widely used to treat fever, pain, inflammation and cerebral infarction in clinic. Panax Notoginseng Saponins (PNS) is the extracts of Panax Notoginseng (PN)-a traditional Chinese medicine extensively used in cardiovascular diseases. Panax notoginseng saponins and ASA are both widely used to treat cerebral infarction in China. Good results in clinical practice have been achieved when the two drugs were taken together. To investigate the effect of PNS on ASA in vivo, the concentrations of salicylic acid (SA) in blood were measured after oral administration of ASA or ASA combined with PNS by UPLC-MS/MS. Sample preparation was carried out by the protein precipitation technique with an internal Saikosaponin A standard. The separation of two components was achieved by using an ACQUITY UPLC (R) BEH C18 Column (1.7 mu m 2.1 x 100 mm) by gradient elution using water (containing 0.2% formic acid) and acetonitrile (containing 0.2% formic acid) as the mobile phase at a flow rate of 0.2 mL/min. The pharmacokinetic parameters were determined by using non-compartmental analysis. The results suggested that drug-drug interaction in vivo existed between PNS and ASA. The concentration of the SA was increasing when the two drugs were administered together. The transport of ASA and SA in MDCK -MDR1 cell monolayer was used to verify this conclusion. The values of apparent permeability coefficients (Papp) were significantly increased when the two drugs were used together. This result suggested PNS could increase the gastrointestinal tract absorption of ASA and SA. These findings provide more insight for wise use of two drugs to treat or prevent cardiovascular diseases. (C) 2016 Elsevier B.V. All rights reserved.