Carbon Nanodots Derived from Urea and Citric Acid in Living Cells: Cellular Uptake and Antioxidation Effect
Carbon Nanodots Derived from Urea and Citric Acid in Living Cells: Cellular Uptake and Antioxidation Effect
复制标题
DOI：
10.1021/acs.langmuir.0c01598
复制
发表时间：
2020-07-28
期刊：
影响因子：
3.9
通讯作者：
Wei, Jianjun
中科院分区：
文献类型：
--
作者：
Ji, Zuowei;Yin, Ziyu;Wei, Jianjun
碳纳米点（CND）被报道为被无定形碳框架包围的多原子碳域，由于其易于合成、出色的电子性能和优异的生物相容性而引起了广泛的关注。然而，考虑到这种相对较新的纳米粒子的复杂性质，仍然需要对其生物性能进行实质性评估。在本报告中，研究了源自尿素和柠檬酸的 CND（U-CND）对两种细胞系 EA.hy926 和 A549 细胞的处理，以检查其生物相容性、细胞摄取和抗氧化效果。细胞内摄取研究表明，进入细胞的能量依赖性转运过程主要涉及巨胞饮作用和脂筏介导的内吞作用途径。此外，U-CND 主要靶向线粒体，并通过清除细胞中的活性氧 (ROS) 表现出强大的抗氧化作用。总体而言，本报告的研究结果表明，U-CND 可以作为生物成像试剂和抗氧化剂，对两种细胞系的活力、质膜完整性和线粒体活性几乎没有危害，并且在较高浓度下对抑制 A549 癌细胞的代谢活动具有一定的功效。
Carbon nanodots (CNDs), reported as polyatomic carbon domains surrounded by amorphous carbon frames, have drawn extensive attention due to their easy-to-synthesis, outstanding electronic properties, and superior biocompatibility. However, substantial assessments regarding their biological performance are still needed, considering the complex nature of this type of relatively new nanoparticles. In this report, CNDs derived from urea and citric acid (U-CNDs) are investigated in the treatment of two cell lines, EA.hy926 and A549 cells, to examine the biocompatibility, cellular uptake, and antioxidation effect. The intracellular uptake study suggests an energy-dependent transport process into the cells mainly involving macropinocytosis and lipid raft-mediated endocytosis pathways. Moreover, the U-CNDs mostly target the mitochondria and present strong antioxidative effects by scavenging reactive oxygen species (ROS) in cells. Overall the findings in this report manifest that the U-CNDs could serve as a bioimaging reagent and antioxidant causing little deleteriousness in the respects of viability, plasma membrane integrity, and mitochondrial activity in both cell lines, and demonstrate some efficacy for inhibiting the metabolic activities of A549 cancer cells at higher concentration.