Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis
Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis
复制标题
克隆哺乳动物肝素酶--肿瘤侵袭和转移过程中的一种重要酶
DOI：
10.1038/10525
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发表时间：
1999-07-01
期刊：
影响因子：
82.9
通讯作者：
Parish, CR
中科院分区：
文献类型：
--
作者：
Hulett, MD;Freeman, C;Parish, CR
糖苷内切酶类肝素酶在入侵细胞，特别是转移性肿瘤细胞和迁移性白细胞降解细胞外基质中是重要的。在这里，我们报告的cDNA序列的人血小板酶，它编码一个独特的蛋白质的543个氨基酸，并在激活的小鼠T细胞和高转移性大鼠腺癌的高度同源序列的识别。此外，乙酰肝素酶mRNA在大鼠肿瘤细胞中的表达与其转移潜能相关。详尽的研究表明，只有一个乙酰肝素酶序列，这与这种酶是哺乳动物组织中占主导地位的内切葡萄糖醛酸酶的想法一致。
The endoglycosidase heparanase is an important in the degradation of the extracellular matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we report the cDNA sequence of the human platelet enzyme, which encodes a unique protein of 543 amino acids, and the identification of highly homologous sequences in activated mouse T cells and in a highly metastatic rat adenocarcinoma. Furthermore, the expression of heparanase mRNA in rat tumor cells correlates with their metastatic potential. Exhaustive studies have shown only one heparanase sequence, consistent with the idea that this enzyme is the dominant endoglucuronidase in mammalian tissues.