TRANSPLANTATION OF FETAL HEMATOPOIETIC STEM-CELLS INUTERO - THE CREATION OF HEMATOPOIETIC CHIMERAS
TRANSPLANTATION OF FETAL HEMATOPOIETIC STEM-CELLS INUTERO - THE CREATION OF HEMATOPOIETIC CHIMERAS
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DOI：
10.1126/science.2874611
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发表时间：
1986-08-15
期刊：
影响因子：
56.9
通讯作者：
ZANJANI, ED
中科院分区：
文献类型：
--
作者：
FLAKE, AW;HARRISON, MR;ZANJANI, ED
将正常的、未成熟的胎儿造血细胞移植到患有先天性血红蛋白病的免疫前胎儿受体中，可以部分重建正常的血红蛋白生成，而不会出现与出生后骨髓移植相关的并发症（免疫抑制和移植物抗宿主病的发生）。为了检验这一假设，自然发生的多态性。将羊血红蛋白位点作为移植和造血嵌合的标记。将同种异体胚胎干细胞腹腔注射到正常的羔羊胎中，4个存活的受体中有3个出现了造血嵌合。这种嵌合现象在出生后6个月和移植后9个月持续存在，没有证据表明存在移植物抗宿主病，也没有使用免疫抑制治疗。
Transplantation of normal, immature, fetal hematopoietic cells into a preimmune fetal recipient with a congenital hemoglobinopathy may allow partial reconstitution of normal hemoglobin production without the complications associated with postnatal bone marrow transplantation (immunosuppression and the occurrence of graft versus host disease). In order to test this hypothesis the naturally occurring polymorphism at the .beta.-hemoglobin locus of the sheep was used as a marker for engraftment and hematopoietic chimerism. Intraperitoneal injection of allogeneic fetal stem cells into normal fetal lambs resulted in hematopoietic chimerism in three of four surviving recipients. This chimerism has been sustained for 6 months after birth and 9 months after engraftment, without evidence of graft versus host disease, and without the use of immunosuppressive therapy.