Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers
Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers
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DOI：
10.1073/pnas.1114551109
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发表时间：
2012-05-29
影响因子：
11.1
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Jayakumar, Muthu Kumara Gnanasammandhan;Idris, Niagara Muhammad;Zhang, Yong
生物分子的受控活化或释放在各种生物应用中是非常关键的。控制生物分子的活性已经尝试了各种手段，并且在过去的十年中，通过光控制活性已经得到普及。该过程中的主要障碍是可光活化化合物主要对紫外线辐射有反应，而对可见光或近红外（NIR）光没有反应。紫外线辐射的使用受到其毒性和非常低的组织穿透能力的限制。在这项研究中，我们报告了近红外-紫外上转换纳米粒子（UCN）的潜力，它作为纳米转换器吸收近红外光具有高的组织穿透能力和可忽略的光毒性和局部发射紫外光，用于笼状化合物的光活化，特别是用于光控基因表达的开发。在溶液和细胞中进行GFP的激活和敲低，并且通过使用由NIR至UV UCN产生的上转换UV光成功地实现了GFP的模式化激活。还完成了通过组织幻影的深度光活化和笼状核酸的体内活化。该方法的成功在分子的光控活化和递送领域中定义了独特的水平。
Controlled activation or release of biomolecules is very crucial in various biological applications. Controlling the activity of biomolecules have been attempted by various means and controlling the activity by light has gained popularity in the past decade. The major hurdle in this process is that photoactivable compounds mostly respond to UV radiation and not to visible or near-infrared (NIR) light. The use of UV irradiation is limited by its toxicity and very low tissue penetration power. In this study, we report the exploitation of the potential of NIR-to-UV upconversion nanoparticles (UCNs), which act as nanotransducers to absorb NIR light having high tissue penetration power and negligible phototoxicity and emit UV light locally, for photoactivation of caged compounds and, in particular, used for photo-controlled gene expression. Both activation and knockdown of GFP was performed in both solution and cells, and patterned activation of GFP was achieved successfully by using upconverted UV light produced by NIR-to-UV UCNs. In-depth photoactivation through tissue phantoms and in vivo activation of caged nucleic acids were also accomplished. The success of this methodology has defined a unique level in the field of photo-controlled activation and delivery of molecules.