Effects of leptin on the release of luteinizing hormone, growth hormone and prolactin from cultured bovine anterior pituitary cells
Effects of leptin on the release of luteinizing hormone, growth hormone and prolactin from cultured bovine anterior pituitary cells
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瘦素对培养牛垂体前叶细胞释放黄体生成素、生长激素和催乳素的影响
DOI：
10.1111/j.1740-0929.2005.00287.x
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发表时间：
2005
影响因子：
2
通讯作者：
E. Kasuya
中科院分区：
文献类型：
--
作者：
S. Nonaka;T. Hashizume;E. Kasuya
研究了瘦素对体外培养的牛垂体前叶（AP）细胞分泌促黄体生成素（LH）、生长激素（GH）和催乳素（PRL）的影响。AP细胞从完全饲喂的日本黑阉牛中获得，在杜氏改良伊格尔培养基中孵育3天后，用10−13至10−7 mol/L的瘦素孵育3小时。与对照组相比，10−8和10−7 mol/L的瘦素分别使培养基中LH的浓度显著增加了45%和44%（P < 0.05）。在10−8和10−7 mol/L的剂量下，瘦素分别使培养基中GH的浓度显著增加14%和12%（P < 0.05）。与对照组相比，10−7 mol/L的瘦素也显著增加了培养基中PRL的浓度26%（P < 0.05）。这些结果表明，瘦素刺激释放LH，GH和PRL直接作用于牛AP细胞从充分喂养的阉牛。
The effects of leptin on the release of luteinizing hormone (LH), growth hormone (GH) and prolactin (PRL) were studied in cultured bovine anterior pituitary (AP) cells in vitro. The AP cells were obtained from fully-fed Japanese Black steers and were incubated for 3 h with 10−13 to 10−7 mol/L of leptin after incubating in Dulbecco's modified Eagle's Medium for 3 days. Leptin significantly increased the concentration of LH in the culture medium by 45 and 44% at doses of 10−8 and 10−7 mol/L, respectively, compared with the controls (P < 0.05). Leptin significantly increased the concentration of GH in the culture medium by 14 and 12% at doses of 10−8 and 10−7 mol/L, respectively (P < 0.05). Leptin also significantly increased the concentration of PRL in the culture medium by 26% compared with the controls at a dose of 10−7 mol/L (P < 0.05). These results show that leptin stimulates the release of LH, GH and PRL by acting directly on bovine AP cells from fully-fed steers.