Preparation and Characterization of Porous Horseradish Peroxidase Microspheres for the Removal of Phenolic Compound and Dye
Preparation and Characterization of Porous Horseradish Peroxidase Microspheres for the Removal of Phenolic Compound and Dye
复制标题
去除酚类化合物和染料的多孔辣根过氧化物酶微球的制备及表征
DOI：
10.1021/ie500302p
复制
发表时间：
2014-05-07
影响因子：
4.2
通讯作者：
Gao, Jing
中科院分区：
文献类型：
--
作者：
Jiang, Yanjun;Cui, Cuicui;Gao, Jing
本文以碳酸钙微粒为模板，采用一种简单、通用、温和的方法制备多孔酶微球。制备过程包括酶与CaCO 3共沉淀、交联和模板去除三个步骤。对制备工艺进行了优化，并对所制备的辣根过氧化物酶（HRP）多孔微球（p-HRP）进行了表征。的p-HRP表现出改善的热稳定性，在碱性条件下比其游离对应物更稳定。这些特性对于实际应用是有吸引力的。与游离HRP相比，p-HRP的Km值增大，Vmax值减小。以苯酚和直接黑38为模型底物，考察了p-HRP对水溶液中酚类化合物和染料的催化去除性能。结果表明，p-HRP具有较高的去除效率和良好的可重复使用性。
In this article, a simple, versatile, and mild method was used to fabricate porous enzyme microspheres using CaCO3 microparticles as templates. The preparation process involved three steps including coprecipitation of enzyme with CaCO3, cross-linking, and template removal. The preparation process was optimized and the obtained porous horseradish peroxidase (HRP) microspheres (p-HRP) were characterized. The p-HRP exhibited improved thermal stability and was more stable under alkali conditions than its free counterpart. These features are attractive for practical applications. Compared to free HRP, the Km value of p-HRP was increased and the Vmax value was decreased. The catalytic performance of p-HRP in removing phenolic compound and dye from aqueous solution was explored by using phenol and Direct Black-38 as model substrates. The results indicated that p-HRP exhibited high removal efficiency and moderately good reusability.