A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus
A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus
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DOI：
10.1073/pnas.0913535107
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发表时间：
2010-05-04
影响因子：
11.1
通讯作者：
Yi, Xianhong
中科院分区：
文献类型：
--
作者：
Yu, Xiao;Li, Bo;Yi, Xianhong
真菌病毒是感染真菌的病毒，当与弱毒力相关时，具有控制作物真菌病害的潜力。通常，真菌病毒具有双链（ds）或单链（ss）RNA基因组。以前没有报道过具有DNA基因组的真菌病毒。在这里，我们描述了一个低致病性相关的环状ssDNA真菌病毒从植物病原真菌核盘菌。该病毒基因组全长2166 nt，编码复制起始蛋白（Rep）和外壳蛋白（CP），命名为核盘菌低致病性相关DNA病毒1（Sclerotinia sclerotiorum hypoxidence-associated DNA virus 1，SsHADV-1）。虽然Rep的系统发育分析表明SsHADV-1与双生病毒相关，但它在基因组组织和颗粒形态上都与双生病毒明显不同。聚乙二醇介导的真菌原生质体的转染是成功的纯化SsHADV-1颗粒或病毒DNA直接从感染的菌丝体分离。ssDNA真菌病毒的发现增强了探索真菌病毒作为真菌分子操作和植物病害控制的有价值工具的潜力，并扩展了我们对全球病毒生态学和进化的了解。
Mycoviruses are viruses that infect fungi and have the potential to control fungal diseases of crops when associated with hypovirulence. Typically, mycoviruses have double-stranded (ds) or single-stranded (ss) RNA genomes. No mycoviruses with DNA genomes have previously been reported. Here, we describe a hypovirulence-associated circular ssDNA mycovirus from the plant pathogenic fungus Sclerotinia sclerotiorum. The genome of this ssDNA virus, named Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), is 2166 nt, coding for a replication initiation protein (Rep) and a coat protein (CP). Although phylogenetic analysis of Rep showed that SsHADV-1 is related to geminiviruses, it is notably distinct from geminiviruses both in genome organization and particle morphology. Polyethylene glycol-mediated transfection of fungal protoplasts was successful with either purified SsHADV-1 particles or viral DNA isolated directly from infected mycelium. The discovery of an ssDNA mycovirus enhances the potential of exploring fungal viruses as valuable tools for molecular manipulation of fungi and for plant disease control and expands our knowledge of global virus ecology and evolution.