Inhibitory Effects of Scutellaria baicalensis Root Extract on Linoleic Acid Hydroperoxide-induced Lung Mitochondrial Lipid Peroxidation and Antioxidant Activities
Inhibitory Effects of Scutellaria baicalensis Root Extract on Linoleic Acid Hydroperoxide-induced Lung Mitochondrial Lipid Peroxidation and Antioxidant Activities
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DOI：
10.3390/molecules24112143
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发表时间：
2019-06-01
期刊：
影响因子：
4.6
通讯作者：
Lee, Ching Kuo
中科院分区：
文献类型：
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作者：
Liau, Pei Ru;Wu, Ming Shun;Lee, Ching Kuo
在本研究中，我们评价了黄芩对自由基肺组织脂质过氧化(LPO)的保护作用。用不同的溶剂制备了黄芩提取液。测定各提取液中总黄酮和总酚的含量，并通过分析亚油酸氢过氧化氢(LHP)诱导的大鼠肺线粒体LPO来评价ROS的损伤保护作用。此外，通过测定二苯基苦味酰肼(DPPH)、过氧化氢、超氧阴离子自由基和羟基自由基的清除能力，并采用金属络合实验测定其体外抗氧化活性。乙酸乙酯(EtOAc)提取物具有较强的清除ROS的能力，并从该提取物中分离纯化了4个化合物：黄芩苷、黄芩素、汉黄素和黄芩素。在大鼠肺线粒体中，黄芩素对LHP诱导的LPO的抑制作用最明显，并用含量最高的EtOAc提取。因此，黄芩素和丹参乙酸乙酯提取物可能具有保护肺线粒体ROS损伤和抑制LHP诱导的LPO的作用。有必要进行更多的研究，以调查它们在呼吸道感染、营养补充剂和药物中的先导化合物中作为抗氧化疗法的用途。
In this study, we evaluated the ability of Scutellaria baicalensis Georgi to protect lipid-peroxidation (LPO) in lung tissue after free radical-induced injury. We prepared S. baicalensis root (SBR) extracts using different solvents. The total flavonoid and total phenol contents of each extract were measured, and the ROS damage protection was evaluated by analyzing linoleic acid hydroperoxide (LHP)-induced LPO in rat lung mitochondria. Moreover, evaluating diphenylpicrylhydrazyl (DPPH), hydrogen peroxide, superoxide anion radical, and hydroxyl radical scavenging abilities and using metal chelating assays were used to determine in vitro antioxidant activity. The ethyl acetate (EtOAc) extract showed high ROS scavenging ability, and four compounds were subsequently isolated and purified from this extract: baicalin, baicalein, wogonin, and oroxylin A. Baicalein in rat lung mitochondria the most significant LHP-induced LPO inhibition was shown and extracted with EtOAc that contained the highest amount of baicalein. Thus, baicalein and the EtOAc extract of SBR may be efficient in conferring ROS damage protection and inhibiting LHP-induced LPO in rat lung mitochondria. Additional studies are warranted to investigate their use as antioxidant therapy for respiration infections, nutrition supplements, and lead compounds in pharmaceuticals.