Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)
Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)
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DOI：
10.1534/genetics.108.097469
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发表时间：
2009-02-01
期刊：
影响因子：
3.3
通讯作者：
Sourdille, Pierre
中科院分区：
文献类型：
--
作者：
Saintenac, Cyrille;Falque, Matthieu;Sourdille, Pierre
在小麦(Triticum aestivum L.)中，从着丝粒到端粒的交叉(CO)频率逐渐增加。然而，人们对影响沿该梯度的复合分布和强度的因素知之甚少。为了研究这一点，我们详细研究了面包小麦3B染色体上的CO模式。将包含102个均匀分布在染色体上的标记的密集参考遗传图谱与物理缺失图谱进行比较。大多数CoS(90%)发生在占染色体40%的远端亚端粒区域。着丝粒周围约27%的近端区域显示出非常弱的CO频率，在所研究的752个配子中只发现了3个CO。此外，我们还观察到短臂远端的CO频率明显降低。最后，首次对小麦中的干扰强度进行了评估。使用伽马模型。结果表明，雄性重组值为1.2，雌性重组值为3.5，表明小麦3B染色体上存在正向干扰。
In wheat (Triticum aestivum L.), the crossover (CO) frequency increases gradually from the centromeres to the telomeres. However, little is known about the factors affecting both the distribution and the intensity of recombination along this gradient. To investigate this, we studied in detail the pattern of CO along chromosome 3B of bread wheat. A dense reference genetic map comprising 102 markers homogeneously distributed along the chromosome was compared to a physical deletion map. Most of the COs (90%) occurred in the distal subtelomeric regions that represent 40% of the chromosome. About 27% of the proximal regions Surrounding the centromere showed a very weak CO frequency with only three COs found in the 752 gametes Studied. Moreover, we observed a clear decrease of CO frequency on the distal region of the short arm. Finally, the intensity of interference was assessed for the first time in wheat. using a Gamma model. The results showed in values of 1.2 for male recombination and 3.5 for female recombination, Suggesting positive interference along wheat chromosome 3B.