A clinical case of enzootic bovine leukosis diagnosed by clonal analysis of peripheral B-cell in a Japanese Black cow.
A clinical case of enzootic bovine leukosis diagnosed by clonal analysis of peripheral B-cell in a Japanese Black cow.
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通过对日本黑牛外周 B 细胞进行克隆分析诊断出的地方性牛白血病临床病例。
DOI：
10.1007/s00580-022-03432-3
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Inokuma;H.;Nagata;T.;Maezawa;M.;Watanabe;K.;Kobayashi;Y.;Kojima;K.;Chambers;JK.;Uchida;K.
一头患有厌食症的7岁日本黑牛被送到当地一家兽医那里。由于直肠触诊显示子宫硬化扩大，怀疑是淋巴瘤，患者被介绍到奥比希罗农业和兽医大学兽医教学医院。虽然未发现淋巴结病和淋巴细胞增多症，但牛白血病病毒(BLV)的前病毒载量很高，达464拷贝/10ngDNA，外周血中偶见有缩窄或花状核的淋巴细胞，因此怀疑是地方性牛白血病(EBL)。根据免疫球蛋白重链(IgH)基因重排的B细胞克隆性分析，外周血B细胞有明显的单克隆性增殖，提示外周血中存在致瘤性B细胞并发生EBL。病理检查包括尸检和组织病理学检查证实了EBL的诊断。外周血中B细胞的克隆性分析被认为对诊断EBL等B细胞肿瘤很有用，特别是在没有淋巴结病和淋巴细胞增多的病例中。这是首例临床诊断为EBL的病例，通过基于IgH基因重排的聚合酶链式反应证实外周血B细胞的单克隆性增殖。
A 7-year-old Japanese Black cow with anorexia was presented at a local veterinarian. As rectal palpation revealed an enlarged induration of the uterus, lymphoma was suspected, and the patient was introduced to the Veterinary Teaching Hospital of Obihiro University of Agriculture and Veterinary Medicine. Although lymphadenopathy and lymphocytosis were not found, a high proviral load of bovine leukemia virus (BLV) with 464 copies/10 ng DNA was recorded, and lymphocytes with constricted or flower-like nuclei were found occasionally in the peripheral blood; accordingly, enzootic bovine leukosis (EBL) was suspected. According to PCR for clonality analysis of B cells based on immunoglobulin heavy chain (IGH) gene rearrangement, monoclonal proliferation of peripheral blood B cells was evident, suggesting the presence of tumorigenic B cells in the peripheral blood and onset of EBL. Pathological examination including necropsy and histopathological examination confirmed the diagnosis of EBL. Clonality analysis for B cells in the peripheral blood is thought to be useful for diagnosing B-cell tumors such as EBL, especially in cases lacking lymphadenopathy and lymphocytosis. This is the first clinical case of EBL diagnosed by demonstrating monoclonal proliferation of peripheral blood B cells using IGH gene rearrangement-based PCR.