SlmiR482e-5p regulates tomato resistance to Phytophthora infestans infection along with slmiR482e-3p via sllncRNA39298-mediated inhibition
SlmiR482e-5p regulates tomato resistance to Phytophthora infestans infection along with slmiR482e-3p via sllncRNA39298-mediated inhibition
复制标题
SlmiR482e-5p 与 slmiR482e-3p 一起通过 sllncRNA39298 介导的抑制调节番茄对致病疫霉感染的抗性
DOI：
10.1016/j.pmpp.2022.101875
复制
发表时间：
2022-08-05
影响因子：
2.7
通讯作者：
Luan, Yushi
中科院分区：
文献类型：
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作者：
Liu, Weiwei;Cui, Jun;Luan, Yushi
SlmiR482e-3p参与番茄与疫霉的相互作用。在这项研究中，我们评估了互补链slmiR482e-5p的功能。在转基因植物中，slMIR482e、slMIR482e -3p和slMIR482e -5p过表达增加了对鼠疫杆菌感染的易感性，而slMIR482e -3p和slMIR482e -5p沉默增加了抗性。此外，sllncRNA39298抑制了slmiR482e-5p的表达，增加了对P. infestans的抗性。感染病原菌后，过表达slMIR482e、slMIR482e -3p和slMIR482e -5p的叶片活性氧积累量高于野生型叶片，过表达sllncrna39298的叶片活性氧积累量低于野生型叶片。这些结果支持了互补链miRNAs的功能重要性，并揭示了slmiR482e-5p和sllncRNA39298在番茄抗病中的作用。
SlmiR482e-3p is involved in tomato-Phytophthora infestans interactions. In this study, we evaluated the function of the complementary strand slmiR482e-5p. In transgenic plants slMIR482e, slmiR482e-3p, and slmiR482e-5p overexpression increased susceptibility to P. infestans infection, while slmiR482e-3p and slmiR482e-5p silencing increased resistance. Additionally, sllncRNA39298 inhibited slmiR482e-5p expression and increased resistance to P. infestans. Upon P. infestans infection, reactive oxygen species accumulation was higher in leaves overexpressing slMIR482e, slmiR482e-3p, and slmiR482e-5p and lower in sllncRNA39298-overexpressing leaves than in wild-type leaves. These results support the functional importance of complementary strand miRNAs and reveal the roles of slmiR482e-5p and sllncRNA39298 in tomato disease resistance.