Comparative Genomic Hybridization Study of Paraffin-Embedded Dedifferentiated Liposarcoma Fixed With Holland Bouin's Fluid
Comparative Genomic Hybridization Study of Paraffin-Embedded Dedifferentiated Liposarcoma Fixed With Holland Bouin's Fluid
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Holland Bouin 液固定石蜡包埋去分化脂肪肉瘤的比较基因组杂交研究
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
A. Aurias
中科院分区：
文献类型：
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作者：
I. Hostein;J. Coindre;J. Derré;O. Mariani;F. Chibon;A. Aurias
去分化脂肪肉瘤和分化脂肪肉瘤以12q15染色体扩增为特征。比较基因组杂交是检测基因组DNA拷贝数变化的有力工具。这项技术已被广泛应用于冰冻肿瘤和福尔马林固定的石蜡包埋肿瘤的一些研究中。本研究的目的是证明当从用Holland Bouin液固定的组织中提取DNA时，CGH能够检测基因组中DNA拷贝数的变化。对16例冷冻和固定在Holland Bouin液中的脂肪肉瘤进行了CGH检查。在冷冻脂肪肉瘤(Amp 12q15、Amp 6q23、Amp 1p32、Amp 16q22、+7、+8)中检测到的主要染色体改变有81%发生在相应的固定肿瘤中。与福尔马林提取的DNA相比，使用Holland Bouin的液体提取的DNA时，这项技术的局限性是可分析样品的产量。用Holland Bouin液固定的肿瘤可分析率为81%(13/16)，而福尔马林固定的肿瘤可分析率为100%(4/4)。这项研究表明，比较基因组杂交是一种有用的工具，即使只有固定的组织(福尔马林和Holland Bouin的液体组织)可用，而且它允许在回顾研究中分析更多的肿瘤。
Dedifferentiated and differentiated liposarcoma are characterized by 12q15 chromosomal amplification. Comparative genomic hybridization is a powerful tool able to detect DNA copy number changes in the genome. This technique has been widely used in frozen tumors and in some studies in paraffin-embedded tumors fixed with formalin. The purpose of this study was to demonstrate the ability of CGH to detect DNA copy number changes in the genome when the DNA was extracted from tissues fixed with Holland Bouin's fluid. Sixteen liposarcoma tumors both frozen and fixed in Holland Bouin's fluid were characterized by CGH. Eighty-one percent of the main chromosomal alterations detected in the frozen liposarcomas (amp 12q15, amp 6q23, amp 1p32, amp 16q22, +7, +8) were detected in the corresponding fixed tumors. The limitation of this technique when using Holland Bouin's fluid extracted DNA compared with formalin-extracted DNA was the yield of analyzable samples. Eighty-one percent of tumors fixed with Holland Bouin's fluid (13/16) were analyzable compared with 100% of formalin-fixed tumors (4/4). This study demonstrates that comparative genomic hybridization is a useful tool even if only fixed tissues (formalin and Holland Bouin's fluid tissues) are available, and that it allows more tumors to be analyzed in retrospective studies.
组织显微切割和简并寡核苷酸引物聚合酶链反应（DOP-PCR）是分析侵袭性肿瘤遗传畸变的有效方法。
DOI：
10.1016/s1525-1578(10)60653-8
发表时间：
2001
期刊：
The Journal of molecular diagnostics : JMD
影响因子：
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作者：
Hirose,Y;Aldape,K;Takahashi,M;Berger,MS;Feuerstein,BG
通讯作者：
Feuerstein,BG