Development of multi-ESI-sprayer, multi-atmospheric-pressure-inlet mass spectrometry and its application to accurate mass measurement using time-of-flight mass spectrometry
Development of multi-ESI-sprayer, multi-atmospheric-pressure-inlet mass spectrometry and its application to accurate mass measurement using time-of-flight mass spectrometry
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DOI：
10.1021/ac990777e
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发表时间：
2000-01-01
影响因子：
7.4
通讯作者：
Moini, M
中科院分区：
文献类型：
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作者：
Jiang, LF;Moini, M
对高质量精度飞行时间质谱仪（TOF-MS）的大气压进样喷嘴（孔板、加热毛细管或进样口）进行了改进，将原来的单喷嘴改为多个大气压进样喷嘴。这允许将多个液体La流并行引入MS（每个喷嘴一个电喷雾器），流之间的分析物相互作用最小。使用单个质谱仪同时分析所有液体流的化学含量。为了通过在每次扫描（采集）时提供内部参考来获得更高的质量准确度，并评价TOF-MS用于分子式确认的适用性，使用了该设计的双ESI喷雾器、双喷嘴版本。测量了200-3000 Da范围内有机化合物的透镜的准确质量，并与双喷雾器、单喷嘴飞行时间质谱法的结果进行了比较。双喷嘴TOF-MS和使用双聚焦质谱仪在化学电离（CI）和快原子轰击（FAB）下操作获得的结果显示了该技术用于元素组成确认的适用性。大约85%的分析样品的质量误差小于5 ppm，另外15%的质量误差小于6 ppm。使用高效液相色谱法（HPLC）作为用于引入一种液体流（样品）的装置，并且使用注射器泵作为用于引入第二种液体流（参比标准）的装置，测量细胞色素c的胰蛋白酶消化物的精确质量。质量误差范围为-6.1 ppm至+3.6 ppm，比我们以前报道的使用单喷嘴飞行时间质谱的质量准确度有了显著的提高。双ESI喷雾器、单喷嘴双ESI喷雾器、Y形入口和双ESI喷雾器、双入口结果证明双ESI喷雾器、双入口设计在每次采集时提供参考峰，具有最小的分析物-参考相互作用，因此具有更高的一致质量精度。
The atmospheric pressure sampling nozzle (orifice, heated capillary, or inlet) of a high mass accuracy time-of-flight mass spectrometer (TOF-MS) was modified by replacing its single nozzle with multiple atmospheric pressure nozzles. This allowed multiple streams of liquids La be introduced into the MS in parallel (an electrosprayer for each nozzle), with minimum analyte interactions between the streams. The chemical contents of all liquid streams were analyzed concurrently using a single mass spectrometer. To obtain a higher mass accuracy by providing internal reference on each scan (acquisition) and to evaluate the suitability of TOF-MS for molecular-formula confirmation, a dual-ESI-sprayer, dual-nozzle version of this design was used. The accurate masses of Lens of organic compounds in the mass range of 200-3000 Da were measured, and the results were compared with those obtained using dual-sprayer, single-nozzle TOF-MS. A significant improvement in mass accuracy was observed when the former technique was used, Comparison between the mass accuracy using dual-ESI-sprayer, dual-nozzle TOF-MS and that obtained using a double-focusing mass spectrometer operating under chemical ionization (CI) and fast atom bombardment (FAB) shows the suitability of the technique for elemental-composition confirmation. Approximately 85% of samples analyzed had mass errors of less than 5 ppm, and the other 15% had mass errors less than 6 ppm. Using a high-performance liquid chromatography (HPLC) as a device for introduction of one liquid stream (sample) and a syringe pump as a device for introduction of the second liquid stream (reference standard), the accurate mass of a tryptic digest of cytochrome c was measured. The range of mass errors was from -6.1 ppm to +3.6 ppm, a significant improvement over our previously reported mass accuracy for this digest using single-nozzle TOF-MS. The interactions between analytes in the liquid streams also were investigated using a variety of sample-introduction and nozzle-design combinations, including single-ESI-sprayer, single-nozzle; dual-ESI-sprayer, single-nozzle- dual-ESI-sprayer, Y-shaped inlet; and dual-ESI-sprayer, dual-inlet The results demonstrated that the dual-ESI-sprayer, dual-inlet design provides reference peaks on every acquisition with minimum analyte-reference interaction and, therefore, higher consistent mass accuracy.