1H and 13C HR-MAS spectroscopy of intact biopsy samples ex vivo and in vivo 1H MRS study of human high grade gliomas
1H and 13C HR-MAS spectroscopy of intact biopsy samples ex vivo and in vivo 1H MRS study of human high grade gliomas
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DOI：
10.1002/nbm.888
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发表时间：
2004-06-01
影响因子：
2.9
通讯作者：
Celda, B
中科院分区：
文献类型：
--
作者：
Martínez-Bisbal, MC;Martí-Bonmatí, L;Celda, B
高分辨率魔角自旋（HR-MAS）一维和二维H-1和C-13核磁共振（NMR）光谱已被用于研究完整的胶质母细胞瘤（GBM）脑肿瘤组织。并与体外化学提取物和体内光谱进行了比较。H-1一维、H-1 TOCSY和C-13 HSQC HR-MAS光谱的分辨率与高氯酸浸提液的分辨率相当。C-13 HSQC HR-MAS光谱对于完整活检肿瘤中37种不同代谢物的鉴定特别有用，不包括水和DSS组分。据我们所知，这是在完整人体组织中获得的生化化合物的最详细的分配，特别是在脑组织中。通过将样品保持在4 ℃的温度下，避免了NMR实验记录期间的组织降解。获得了10个GBM（6个原发性，2个继发性和2个未分类）的详细代谢组成。已发现离体和体内MRS之间的良好相关性。版权所有（C）2004 John Wiley Sons，Ltd.
High-resolution magic angle spinning (HR-MAS) one- and two-dimensional H-1 and C-13 nuclear magnetic resonance (NMR) spectroscopy has been used to study intact glioblastoma (GBM) brain tumour tissue. The results were compared with in vitro chemical extract and in vivo spectra. The resolution of H-1 one-dimensional, H-1 TOCSY and C-13 HSQC HR-MAS spectra is comparable to that obtained on perchloric extracts. C-13 HSQC HR-MAS spectra have been particularly useful for the identification of 37 different metabolites in intact biopsy tumours, excluding water and DSS components. To our knowledge, this is the most detailed assignment of biochemical compounds obtained in intact human tissue, in particular in brain turnout tissue. Tissue degradation during the recording of the NMR experiment was avoided by keeping the sample at a temperature of 4 degreesC. Detailed metabolical compositions of 10 GBM (six primary, two secondary and two unclassified) were obtained. A good correlation between ex vivo and in vivo MRS has been found. Copyright (C) 2004 John Wiley Sons, Ltd.