Selective Manipulation of Biomolecules with Insulator-Based Dielectrophoretic Tweezers.
Selective Manipulation of Biomolecules with Insulator-Based Dielectrophoretic Tweezers.
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DOI：
10.1021/acsanm.9b02302
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发表时间：
2020-01
影响因子：
5.9
通讯作者：
Myungkeun Oh;Vidura Jayasooriya;S. Woo;D. Nawarathna;Yongki Choi
中科院分区：
文献类型：
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作者：
Myungkeun Oh;Vidura Jayasooriya;S. Woo;D. Nawarathna;Yongki Choi
基于绝缘体的介电泳法(IDEP)捕获、分离和浓缩纳米级物体是使用非金属的、无偏压的、可移动的尖端作为镊子进行的。对荧光标记的聚苯乙烯颗粒和DNA进行了空间控制和操纵，以证明IDEP镊子的可行性。用DNA的两种理论方法定量地确定了频率相关的IDEP镊子的强度和极性，它们之间的差异为2~40倍。在任何一种方法中，IDEP的强度都至少比热力强4个数量级，这表明IDEP是捕获、保持和分离DNA的主导力量。还确定了IDEP镊子的捕获强度和体积，进一步支持在末端直接捕获和操纵DNA。
Insulator-based dielectrophoretic (iDEP) trapping, separating, and concentrating nanoscale objects is carried out using a non-metal, unbiased, mobile tip acing as a tweezers. The spatial control and manipulation of fluorescently-labeled polystyrene particles and DNA were performed to demonstrate the feasibility of the iDEP tweezers. Frequency-dependent iDEP tweezers' strength and polarity were quantitatively determined using two theoretical approaches to DNA, which resulted in a factor of 2 ~ 40 differences between them. In either approach, the strength of iDEP was at least 4-order of magnitude stronger than the thermal force, indicating iDEP was a dominant force for trapping, holding, and separating DNA. The trapping strength and volume of the iDEP tweezers were also determined, which further supports direct capture and manipulation of DNA at the tip end.