High Efficiency of Functional Carbon Nanodots as Entry Inhibitors of Herpes Simplex Virus Type 1
High Efficiency of Functional Carbon Nanodots as Entry Inhibitors of Herpes Simplex Virus Type 1
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DOI：
10.1021/acsami.6b01681
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发表时间：
2016-04-13
影响因子：
9.5
通讯作者：
Szunerits, Sabine
中科院分区：
文献类型：
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作者：
Barras, Alexandre;Pagneux, Quentin;Szunerits, Sabine
纳米结构最近被认为是调节病毒附着和进入的有效治疗策略。纳米结构上存在的高浓度配体可以显着增强对生物受体的亲和力。我们在这里展示了用硼酸或胺功能进行表面功能化的碳纳米点 (C-dots) 干扰 1 型单纯疱疹病毒 (HSV-1) 进入的潜力。使用改进的水热碳化法由 4-氨基苯硼酸盐酸盐（4-AB/C-dots）形成的碳点被证明可以在纳克每毫升浓度范围内预防 HSV-1 感染（Vero 和 A549 细胞上的 EC50 分别为 80 和 145 ng mL(-1)），而由苯硼酸形成的相应碳点（B/C-点）即使在高浓度下也没有效果。一些提出的结果还表明，碳点通过与病毒以及可能与细胞同时相互作用，专门作用于病毒进入的早期阶段。
Nanostructures have been lately identified as an efficient therapeutic strategy to modulate viral attachment and entry. The high concentrations of ligands present on nanostructures can considerably enhance affinities toward biological receptors. We demonstrate here the potential of carbon nanodots (C-dots) surface-functionalized with boronic acid or amine functions to interfere with the entry of herpes simplex virus type 1 (HSV-1). C-dots formed from 4-aminophenylboronic acid hydrochloride (4-AB/C-dots) using a modified hydrothermal carbonization are shown to prevent HSV-1 infection in the nanograms per milliliter concentration range (EC50 = 80 and 145 ng mL(-1) on Vero and A549 cells, respectively), whereas the corresponding C-dots formed from phenylboronic acid (B/C-dots) have no effects even at high concentrations. Some of the presented results also suggest that C-dots are specifically acting on the early stage of virus entry through an interaction with the virus and probably the cells at the same time.