Inflammatory Cell Upregulation of the Lid Wiper in Contact Lens Dry Eye
Inflammatory Cell Upregulation of the Lid Wiper in Contact Lens Dry Eye
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DOI：
10.1097/opx.0000000000000865
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发表时间：
2016-08-01
影响因子：
1.4
通讯作者：
Efron, Nathan
中科院分区：
文献类型：
--
作者：
Alzahrani, Yahya;Colorado, Luisa;Efron, Nathan
目的.确定角膜接触镜诱导的干眼症（CLIDE）中眼睑擦拭器中的朗格汉斯细胞是否上调。在日抛型水凝胶角膜接触镜（Biomedics 1 day Extra）配戴6个月后，使用Heidelberg激光扫描共聚焦显微镜检查了17名CLIDE参与者（使用CLDEQ-8评估）和29名无CLIDE（NO-CLIDE）参与者的一只眼睛的眼睑刮片。还检查了20名未配戴接触镜的对照。每个参与者的朗格汉斯细胞密度（LCD）是从六个清晰的、随机选择的已知尺寸的图像中手动计算的平均细胞计数。三组间眼睑擦拭器的LCD总体差异有统计学意义（p < 0.001）。与对照组（8 +/- 4 cells/mm 2）相比，CLIDE组（17 +/- 10 cells/mm 2）的眼睑擦拭器LCD显著更大（p < 0.001）;然而，NO-CLIDE组（10 +/- 5 cells/mm 2）和对照组之间的LCD无差异（p = 0.489）。CLIDE组的LCD显著大于NO-CLIDE组（p = 0.002）。眼睑擦拭器中的朗格汉斯细胞在CLIDE中上调，表明这种情况的病因学中的炎性成分。
Purpose. To determine if Langerhans cells in the lid wiper are upregulated in contact lens-induced dry eye (CLIDE).Methods. The lid wiper of one eye of 17 participants with CLIDE (assessed using the CLDEQ-8) and 29 without CLIDE (NO-CLIDE) was examined using a Heidelberg laser scanning confocal microscope after 6 months wear of daily disposable hydrogel contact lenses (Biomedics 1 day Extra). Twenty non-contact-lens-wearing controls were also examined. Langerhans cell density (LCD) in each participant was taken as the mean cell count calculated manually from six clear, randomly selected images of known dimensions.Results. There were significant overall differences in LCD in the lid wiper among the three groups (p < 0.001). LCD was significantly greater in the lid wiper in CLIDE (17 +/- 10 cells/mm(2)) compared to controls (8 +/- 4 cells/mm2) (p < 0.001); however, there was no difference in LCD between NO-CLIDE (10 +/- 5 cells/mm(2)) and controls (p = 0.489). LCD was significantly greater in CLIDE than NO-CLIDE (p = 0.002).Conclusions. Langerhans cells in the lid wiper are upregulated in CLIDE, suggesting an inflammatory component in the etiology of this condition.