Genomic inventory and expression of Sox and Fox genes in the cnidarian Nematostella vectensis
Genomic inventory and expression of Sox and Fox genes in the cnidarian Nematostella vectensis
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DOI：
10.1007/s00427-005-0022-y
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发表时间：
2005-12-01
影响因子：
2.4
通讯作者：
Martindale, MQ
中科院分区：
文献类型：
--
作者：
Magie, CR;Pang, K;Martindale, MQ
Sox 和 Forkhead (Fox) 基因家族由转录因子组成，它们在各种发育过程中发挥重要作用，包括胚层规范、原肠胚形成、细胞命运决定和形态发生。 Sox 和 Fox 基因家族都根据各自 DNA 结合结构域、高迁移率组 (HMG) 盒（Sox 基因）或叉头结构域（Fox 基因）的氨基酸序列分为亚组。利用刺胞动物 Nematostella vectensis 的基因组草图，我们检查了该生物体中 Sox 和 Fox 基因的基因组互补，以深入了解这些基因家族在基础后生动物中的性质。我们在 Nematostella 中鉴定了 14 个 Sox 基因和 15 个 Fox 基因，并进行了贝叶斯系统发育分析，比较了来自不同分类单元的 Nematostella 的 HMG box 和 Forkhead 结构域序列。我们发现大多数两侧对称的 Sox 群体具有明确的 Nematostella 直系同源物，而只有少数 Fox 群体具有代表性，这表明驱动这些基因家族多样化的进化压力可能彼此不同。此外，我们检查了这些基因的子集在 Nematostella 发育过程中的表达，发现其中一些基因的表达模式与胚层规范中的作用以及对原肠胚形成重要的细胞行为的调节一致。线虫属这些基因家族成员之间表达模式的多样性强化了这样一种观点，即尽管刺胞动物的形态相对简单，但它们具有在两侧对称动物类群中观察到的大部分分子复杂性。
The Sox and Forkhead (Fox) gene families are comprised of transcription factors that play important roles in a variety of developmental processes, including germ layer specification, gastrulation, cell fate determination, and morphogenesis. Both the Sox and Fox gene families are divided into subgroups based on the amino acid sequence of their respective DNA-binding domains, the high-mobility group (HMG) box (Sox genes) or Forkhead domain (Fox genes). Utilizing the draft genome sequence of the cnidarian Nematostella vectensis, we examined the genomic complement of Sox and Fox genes in this organism to gain insight into the nature of these gene families in a basal metazoan. We identified 14 Sox genes and 15 Fox genes in Nematostella and conducted a Bayesian phylogenetic analysis comparing HMG box and Forkhead domain sequences from Nematostella with diverse taxa. We found that the majority of bilaterian Sox groups have clear Nematostella orthologs, while only a minority of Fox groups are represented, suggesting that the evolutionary pressures driving the diversification of these gene families may be distinct from one another. In addition, we examined the expression of a subset of these genes during development in Nematostella and found that some of these genes are expressed in patterns consistent with roles in germ layer specification and the regulation of cellular behaviors important for gastrulation. The diversity of expression patterns among members of these gene families in Nematostella reinforces the notion that despite their relatively simple morphology, cnidarians possess much of the molecular complexity observed in bilaterian taxa.