Immunodeficient NOD-scid IL-2Rγnull Mice Do Not Display T and B Cell Leakiness
Immunodeficient NOD-scid IL-2Rγnull Mice Do Not Display T and B Cell Leakiness
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DOI：
10.1538/expanim.60.181
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发表时间：
2011-04-01
影响因子：
2.4
通讯作者：
Ito, Mamoru
中科院分区：
文献类型：
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作者：
Katano, Ikumi;Ito, Ryoji;Ito, Mamoru
通过回交交配将 IL-2R gamma KO 小鼠的 IL-2R gamma(null) 基因导入 NOD/Shi-scid 小鼠而建立的 NOD/Shi-scid IL-2R gamma(null) (NOG) 小鼠显示出高异种移植植入水平，因此非常适合作为人源化小鼠模型。携带 Prkdc(scid) 基因的 SCID 小鼠表现出胸腺淋巴瘤的高发病率和渗漏现象，即老年小鼠中出现少量克隆性 T 细胞和 B 细胞。在本研究中，评估了 NOG 小鼠是否存在渗漏现象，例如在 C.B-17-scid 和 NOD-scid 小鼠中观察到的渗漏现象。与 C.B-17-scid 和 NOD-scid 小鼠中的发现不同，NOG 小鼠中的血清免疫球蛋白分析未检测到 IgG 或 IgM。流式细胞术分析显示NOG小鼠的外周血和脾脏中不存在T细胞和B细胞。这些结果反映了通过 IL-2R γ 基因的失活抑制了 NOG 小鼠的渗漏现象，该基因通常在 IL-2、IL-4 和 IL-7 的 T 和 B 细胞生长因子受体中表达。
NOD/Shi-scid IL-2R gamma(null) (NOG) mice established by introducing the IL-2R gamma(null) gene of IL-2R gamma KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdc(scid) gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2R gamma gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.