Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene
Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene
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DOI：
10.1016/s1535-6108(03)00054-0
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发表时间：
2003-03-01
期刊：
影响因子：
50.3
通讯作者：
Powers, S
中科院分区：
文献类型：
--
作者：
Mu, D;Chen, LY;Powers, S
使用人类乳腺癌的代表性差异分析 (RDA) 发现了位于染色体区域 8q24.3 的新扩增子。我们检查了一系列含有该区域扩增的乳腺癌样本，并确定 KCNK9 是扩增中心内唯一过表达的基因。 KCNK9 编码的钾通道在 10% 的乳腺肿瘤中扩增 3 倍至 10 倍，在 44% 的乳腺肿瘤中过度表达 5 倍至 100 倍以上。 KCNK9 在细胞系中的过度表达可促进肿瘤形成并赋予对缺氧和血清剥夺的抵抗力，表明其扩增和过度表达在人类乳腺癌中发挥着重要的生理作用。
Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.