Molecular Characteristics of Staphylococcus aureus Causing Bovine Mastitis between 2014 and 2015.
Molecular Characteristics of Staphylococcus aureus Causing Bovine Mastitis between 2014 and 2015.
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2014-2015年牛乳腺炎金黄色葡萄球菌分子特征分析
DOI：
10.3389/fcimb.2017.00127
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发表时间：
2017
影响因子：
5.7
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Li T;Lu H;Wang X;Gao Q;Dai Y;Shang J;Li M
金黄色葡萄球菌具有高致病性，可引起人类和家畜疾病。在包括反刍动物在内的动物物种中，金黄色葡萄球菌可能引起严重或亚临床乳腺炎。本研究旨在调查2014年至2015年中国上海和浙江地区牛乳腺炎病例中分离的212株金黄色葡萄球菌分离株的分子谱、抗菌药物耐药性和基因型/表型相关性。通过多位点序列分型确定了 19 种序列类型（ST），其中占主导地位的 ST 是 ST97，其次是 ST520、ST188、ST398、ST7 和 ST9。在 14 个耐甲氧西林金黄色葡萄球菌 (MRSA) 分离株和 198 个甲氧西林敏感金黄色葡萄球菌 (MSSA) 分离株中，ST97 是主要的 MSSA 克隆，ST9-MRSA-SCCmecXII-spa t899 是最常见的 MRSA 克隆。 MRSA 菌株对多种抗生素的耐药率比 MSSA 菌株高得多。与其他MSSA菌株相比，MSSA ST398对克林霉素、红霉素和环丙沙星的耐药性更强。没有分离株对万古霉素、替考拉宁或利奈唑胺耐药。不同菌株的毒力基因的分子谱有所不同。 ST520菌株携带seg-sei-sem-sen-seo基因，ST9和ST97菌株携带sdrD-sdrE基因。毒力表型分析显示不同克隆的多样性。 ST188和ST7的生物膜形成能力显着增强，除ST7外的所有动物源金黄色葡萄球菌菌株的红细胞裂解能力均较强。我们的结果表明，MSSA 是引起中国东部地区牛乳腺炎的主要金黄色葡萄球菌菌株。然而，多重耐药和产毒MRSA克隆ST9的存在表明，在畜牧产品管理中应对金黄色葡萄球菌感染进行全面监测。
Staphylococcus aureus is highly pathogenic and can cause diseases in both humans and domestic animals. In animal species, including ruminants, S. aureus may cause severe or sub-clinical mastitis. This study aimed to investigate the molecular profile, antimicrobial resistance, and genotype/phenotype correlation of 212 S. aureus isolates recovered from cases of bovine mastitis from 2014 to 2015 in the Shanghai and Zhejiang areas of China. Nineteen sequence types (STs) were determined by multi-locus sequence typing, while the dominant ST was ST97, followed by ST520, ST188, ST398, ST7, and ST9. Within 14 methicillin-resistant S. aureus (MRSA) isolates and 198 methicillin-susceptible S. aureus (MSSA) isolates, ST97 was the predominant MSSA clone and ST9-MRSA-SCCmecXII-spa t899 was the most common MRSA clone. The MRSA strains showed much higher rates of resistance to multiple antibiotics than did MSSA strains. Compared with other MSSA strains, MSSA ST398 was more resistant to clindamycin, erythromycin, and ciprofloxacin. No isolates were resistant to vancomycin, teicoplanin, or linezolid. The molecular profiles of the virulence genes varied in different strains. ST520 strains carried seg-sei-sem-sen-seo genes, and ST9 and ST97 harbored sdrD-sdrE genes. Virulence phenotype analysis showed diversity in different clones. Biofilm formation ability was significantly enhanced in ST188 and ST7, and red blood cell lysis capacity was relatively strong in all S. aureus strains of animal origin except ST7. Our results indicate that MSSA was the predominant S. aureus strain causing bovine mastitis in eastern regions of China. However, the presence of multidrug resistant and toxigenic MRSA clone ST9 suggests that comprehensive surveillance of S. aureus infection should be implemented in the management of animal husbandry products.