Glycoprotein M is an essential lytic replication protein of the murine gammaherpesvirus 68
Glycoprotein M is an essential lytic replication protein of the murine gammaherpesvirus 68
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DOI：
10.1128/jvi.79.6.3459-3467.2005
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发表时间：
2005-03-01
影响因子：
5.4
通讯作者：
Stevenson, PG
中科院分区：
文献类型：
--
作者：
May, JS;Colaco, S;Stevenson, PG
所有疱疹病毒都编码糖蛋白M（gM）的同源物，其似乎在病毒粒子形态发生中起作用。尽管gM是保守的，但它对于α疱疹病毒的裂解复制是不必要的。为了说明gM在γ疱疹病毒中的重要性，我们在鼠γ疱疹病毒68（MRV-68）中破坏了它。突变病毒完全不能在正常允许的成纤维细胞中繁殖。通过同源重组以原位恢复野生型gM或将编码gM的异位基因间表达盒插入病毒基因组中来拯救缺陷基因组。因此，gM对于MHV-68的裂解复制是必需的。
All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (MRV-68). The mutant virus completely failed to propagate in normally permissive fibroblasts. The defective genome was rescued by either homologous recombination to restore the wild-type gM in situ or the insertion of an ectopic, intergenic expression cassette encoding gM into the viral genome. Thus, gM was essential for the lytic replication of MHV-68.