Genetic and Physiological Diversity in the Diatom Nitzschia inconspicua
Genetic and Physiological Diversity in the Diatom Nitzschia inconspicua
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硅藻不起眼菱形藻的遗传和生理多样性
DOI：
10.1111/jeu.12240
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发表时间：
2015
影响因子：
2.2
通讯作者：
Ibanez C & DG Mann
中科院分区：
文献类型：
--
作者：
Rovira R;Trobajo R;Sato S;Ibanez C & DG Mann
耐盐硅藻（Nitzschia in）是一种生态上重要的硅藻种，分布广泛，耐盐，耐有机或营养物污染。然而，它的鉴定并不简单，也没有关于该物种遗传和生态生理多样性的信息。我们利用形态学、分子（rbcL和LSU D1-D3）、生态生理和生殖数据来研究它们是否存在。隐蔽物种由具有广泛生态耐受性的单一物种或具有共同或不同生态偏好的两个或多个隐蔽物种组成。来自埃布罗河流域上游和三角洲遗址的克隆分子遗传数据显示7n。rbcL + LSU基因型分为三个主要分支。其中两个分支与其他nitzschiaanddenticula物种有亲缘关系，使n。不明显过敏性，提示需要进行分类修订。大多数克隆被观察到是自动的，表现出童婚，因此生物物种的概念不能用来建立物种边界。虽然克隆之间存在形态差异，但我们发现不同枝的基因型之间没有一致的差异，这些差异只能通过序列数据来确定。然而，分离基因型可能对生态目的很重要，因为它们之间会遇到两种不同的生态生理反应。
Nitzschia inconspicuais an ecologically important diatom species, which is believed to have a widespread distribution and to be tolerant to salinity and to organic or nutrient pollution. However, its identification is not straightforward and there is no information on genetic and ecophysiological diversity within the species. We used morphological, molecular (rbcL and LSU D1–D3), ecophysiological and reproductive data to investigate whetherN. inconspicuaconstitutes a single species with a broad ecological tolerance or two or more cryptic species with shared or different ecological preferences. Molecular genetic data for clones from upstream and deltaic sites in the Ebro River basin (Catalonia, Spain) revealed sevenN. inconspicua rbcL + LSU genotypes grouped into three major clades. Two of the clades were related to otherNitzschiaandDenticulaspecies, makingN. inconspicuaparaphyletic and suggesting the need for taxonomic revision. Most clones were observed to be automictic, exhibiting paedogamy, and so the biological species concept cannot be used to establish species boundaries. Although there were morphological differences among clones, we found no consistent differences among genotypes belonging to different clades, which are definable only through sequence data. Nevertheless, separating the genotypes could be important for ecological purposes because two different ecophysiological responses were encountered among them.